ARG54001

anti-OCT4 antibody

anti-OCT4 antibody for Western blot and Mouse

Developmental Biology antibody; Gene Regulation antibody

Overview

Product Description

Mouse Monoclonal antibody recognizes OCT3/OCT4

Tested Reactivity Ms
Tested Application WB
Host Mouse
Clonality Monoclonal
Isotype IgG2b
Target Name OCT4
Antigen Species Mouse
Immunogen Purified recombinant mouse POU5F1/OCT4 protein fragments expressed in E.coli.
Conjugation Un-conjugated
Alternate Names OTF-3; OCT4; OCT3; Octamer-binding protein 4; OTF3; Oct-3; Oct-4; OTF4; POU domain, class 5, transcription factor 1; Octamer-binding transcription factor 3; Octamer-binding protein 3

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purified
Buffer 0.1M Tris-Glycine (pH 7.4), 150 mM NaCl, 0.2% Sodium azide and 50% Glycerol
Preservative 0.2% Sodium azide
Stabilizer 50% Glycerol
Concentration Lot dependent
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 18999 Mouse POU5F1

Swiss-port # P20263 Mouse POU domain, class 5, transcription factor 1

Gene Symbol Pou5f1
Gene Full Name POU domain, class 5, transcription factor 1
Background Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3').Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1,FGF4,UTF1 and ZFP206.Critical for early embryogenesis and for embryonic stem cell pluripotency.
Function Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. [UniProt]
Cellular Localization Nucleus.
Research Area Developmental Biology antibody; Gene Regulation antibody
Calculated MW 39 kDa
PTM Sumoylation enhances the protein stability, DNA binding and transactivation activity. Sumoylation is required for enhanced YES1 expression (By similarity).
Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination by WWP2 leading to proteasomal degradation.
ERK1/2-mediated phosphorylation at Ser-111 promotes nuclear exclusion and proteasomal degradation. Phosphorylation at Thr-235 and Ser-236 decrease DNA-binding and alters ability to activate transcription.

Images (1) Click the Picture to Zoom In

  • ARG54001 anti-OCT4 antibody WB image

    Western blot: F9 cell lysate stained with ARG54001 anti-OCT4 antibody at 1:1000 dilution.