ARG45698
anti-ORC5L antibody
anti-ORC5L antibody for Flow cytometry,ICC/IF,Western blot and Human,Mouse,Rat,Monkey
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ORC5L |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Mk |
| Tested Application | FACS, ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ORC5L |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to middle region of human ORC5L. |
| Conjugation | Un-conjugated |
| Alternate Names | ORC5; Origin Recognition Complex Subunit 5; PPP1R117; Orc5p; ORC5T; ORC5L; Protein Phosphatase 1, Regulatory Subunit 117; Origin Recognition Complex, Subunit 5 (Yeast Homolog)-Like; Origin Recognition Complex, Subunit 5 Homolog (Yeast); Origin Recognition Complex, Subunit 5-Like (Yeast); Origin Recognition Complex, Subunit 5 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Observed Size | 50 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O43913 Human Origin recognition complex subunit 5 Swiss-port # Q9WUV0 Mouse Origin recognition complex subunit 5 |
|---|---|
| Gene Symbol | ORC5 |
| Gene Full Name | Origin Recognition Complex Subunit 5 |
| Background | The origin recognition complex (ORC) is a highly conserved six subunit protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. The protein encoded by this gene is a subunit of the ORC complex. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Oct 2010] |
| Function | Component of the origin recognition complex (ORC) that binds origins of replication. DNA-binding is ATP-dependent. The specific DNA sequences that define origins of replication have not been identified yet. ORC is required to assemble the pre-replication complex necessary to initiate DNA replication. [UniProt] |
| Cellular Localization | Chromosome; Nucleus. [UniProt] |
| Calculated MW | 50 kDa |
| PTM | Ubl conjugation. [UniProt] |
Images (6) Click the Picture to Zoom In
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ARG45698 anti-ORC5L antibody ICC/IF image
Immunofluorescence: MCF-7 stained with ARG45698 anti-ORC5L antibody at 5 μg/ml dilution.
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ARG45698 anti-ORC5L antibody WB image
Western blot: MCF-7 stained with ARG45698 anti-ORC5L antibody at 0.5 μg/ml dilution.
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ARG45698 anti-ORC5L antibody FACS image
Flow Cytometry: U87 stained with ARG45698 anti-ORC5L antibody at 1 µg/10^6 cells dilution.
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ARG45698 anti-ORC5L antibody WB image
Western blot: Rat testis stained with ARG45698 anti-ORC5L antibody at 0.5 μg/ml dilution.
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ARG45698 anti-ORC5L antibody WB image
Western blot: Mouse testis stained with ARG45698 anti-ORC5L antibody at 0.5 μg/ml dilution.
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ARG45698 anti-ORC5L antibody WB image
Western blot: COS-7 stained with ARG45698 anti-ORC5L antibody at 0.5 μg/ml dilution.
