anti-Occludin antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Signaling Transduction antibody
Rabbit Polyclonal antibody recognizes Occludin
|Tested Reactivity||Hu, Ms|
|Tested Application||IHC-P, WB|
|Immunogen||Synthetic peptide corresponding to C-terminus of human occludin.|
|Alternate Names||PPP1R115; BLCPMG; Occludin|
|Application Note||IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Incubation Time: 30 min at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Positive Control||Lung, Human Kidney|
|Calculated MW||65 kDa|
|Purification||Immunogen affinity purified|
|Buffer||PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide|
|Preservative||< 0.1% Sodium azide|
|Storage instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||occludin|
|Background||This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5. [provided by RefSeq, Apr 2011]|
|Function||May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions. [UniProt]|
Occludin antibodies; Occludin Duos / Panels; Anti-Rabbit IgG secondary antibodies;
|Research Area||Signaling Transduction antibody|
|PTM||Dephosphorylated by PTPRJ. The tyrosine phosphorylation on Tyr-398 and Tyr-402 reduces its ability to interact with TJP1. Phosphorylation at Ser-490 also attenuates the interaction with TJP1.|
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