ARG53386

anti-Occludin antibody

anti-Occludin antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse

Signaling Transduction antibody
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anti-Occludin antibody

Rabbit Polyclonal antibody recognizes Occludin

ARG53386 Arigo biolaboratories
3.5 out of 5 based on 2
anti-Occludin antibody is rated 3.5 out of 5 by 2.

anti-Occludin antibody

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Overview

Product Description

Rabbit Polyclonal antibody recognizes Occludin

Tested Reactivity Hu, Ms
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Occludin
Antigen Species Human
Immunogen Synthetic peptide corresponding to C-terminus of human occludin.
Conjugation Un-conjugated
Full Name occludin
Alternate Names PPP1R115; BLCPMG; Occludin

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50
WB1:25 - 1:500
Application Note IHC-P: Antigen Retrieval: Boil tissue section in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.
Incubation Time: 30 min at RT.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Lung, Human Kidney
Calculated MW 65 kDa

Properties

Form Liquid
Purification Immunogen affinity purified
Buffer PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide
Preservative < 0.1% Sodium azide
Stabilizer 1% BSA
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 100506658 Human OCLN

GeneID: 18260 Mouse OCLN

Swiss-port # Q16625 Human Occludin

Swiss-port # Q61146 Mouse Occludin

Gene Symbol OCLN
Gene Full Name occludin
Background This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5. [provided by RefSeq, Apr 2011]
Function May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions. [UniProt]
Cellular Localization Membrane
Highlight Related products:
Occludin antibodies; Occludin Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Research Area Signaling Transduction antibody
PTM Dephosphorylated by PTPRJ. The tyrosine phosphorylation on Tyr-398 and Tyr-402 reduces its ability to interact with TJP1. Phosphorylation at Ser-490 also attenuates the interaction with TJP1.

Images (2) Click the Picture to Zoom In

  • ARG53386 anti-Occludin antibody WB image

    Western Blot: Kidney stained with Occludin antibody (ARG53386)

  • ARG53386 anti-Occludin antibody IHC-P image

    Immunohistochemistry: Human Lung stained with Occludin antibody (ARG53386)

Customer's Feedback

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Review for anti-Occludin antibody

Application:WB

Sample:HeLa

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:500

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

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nuts_pic      Good

Review for anti-Occludin antibody

Application:WB

Sample:Mouse kidney

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:100

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

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Specific References

Meningitic Escherichia coli-induced upregulation of PDGF-B and ICAM-1 aggravates blood-brain barrier disruption and neuroinflammatory response.

WB, IHC-P / Human, Mouse

Yang Rui-Cheng et al.
J Neuroinflammation.,  (2019)

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Induction of VEGFA and Snail-1 by meningitic Escherichia coli mediates disruption of the blood-brain barrier.

IHC-P / Mouse

Yang R et al.
Oncotarget., 63839 - 63855 (2016)

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