anti-Olig 2 antibody
anti-Olig 2 antibody for ICC/IF,IHC-Frozen sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
Developmental Biology antibody; Gene Regulation antibody; Neuroscience antibody
|Product Description||Rabbit Polyclonal antibody recognizes Olig 2|
|Tested Reactivity||Hu, Ms, Rat|
|Tested Application||ICC/IF, IHC-Fr, IP, WB|
|Target Name||Olig 2|
|Immunogen||Recombinant mouse Olig2|
|Alternate Names||bHLHb1; Class E basic helix-loop-helix protein 19; Class B basic helix-loop-helix protein 1; Oligo2; bHLHe19; Oligodendrocyte transcription factor 2; BHLHB1; Protein kinase C-binding protein 2; OLIGO2; Protein kinase C-binding protein RACK17; PRKCBP2; RACK17|
|Application Note||Specific for the ~32 kDa Olig2 protein in Western blots. The antibody also works well for immunohistochemistry and immunocytochemistry.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Observed Size||~ 32 kDa|
|Purification||Protein A purified|
|Buffer||100 mM Glycine (pH 8.0), 200 mM Tris|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||oligodendrocyte transcription factor 2|
|Background||Olig2 is a transcription factor which is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Expressed in the ventral spinal cord as early as 9.5 dpc and scattered in the mantle zone, likely corresponding to oligodendrocyte progenitors migrating out from their site of origin. From 10.5 through 14.5 dpc, Olig2 is expressed in numerous cells in the ventricular and subventricular zones of the lateral and medial ganglionic eminences, suggesting that expression might not be limited to the oligodendrocytic lineage. Olig2 has further been shown to be critical in the proliferation of malignant glioma in brain tumors (Ligon et al., 2007).|
|Research Area||Developmental Biology antibody; Gene Regulation antibody; Neuroscience antibody|
|Calculated MW||32 kDa|
Images (2) Click the Picture to Zoom In
ARG52380 anti-Olig 2 antibody ICC/IF image
Immunofluorescence: Primary rat cortical neuroepithelial cells stained with ARG52380 anti-Olig 2 antibody (red). The cells were treated with basic FGF for 30 hours prior to staining (this induces Olig 2 expression in these primary cells).
ARG52380 anti-Olig 2 antibody WB image
Western blot: Neonatal rat brain lysate stained with ARG52380 anti-Olig 2 antibody.