anti-Olig 2 antibody

anti-Olig 2 antibody for ICC/IF,IHC-Frozen sections,Immunoprecipitation,Western blot and Human,Mouse,Rat

Developmental Biology antibody; Gene Regulation antibody; Neuroscience antibody


Product Description Rabbit Polyclonal antibody recognizes Olig 2
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-Fr, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Olig 2
Antigen Species Mouse
Immunogen Recombinant mouse Olig2
Conjugation Un-conjugated
Alternate Names bHLHb1; Class E basic helix-loop-helix protein 19; Class B basic helix-loop-helix protein 1; Oligo2; bHLHe19; Oligodendrocyte transcription factor 2; BHLHB1; Protein kinase C-binding protein 2; OLIGO2; Protein kinase C-binding protein RACK17; PRKCBP2; RACK17

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:500 - 1:1000
IHC-Fr1:500 - 1:1000
IP1:500 - 1:1000
WB1:1000 - 1:3000
Application Note Specific for the ~32 kDa Olig2 protein in Western blots. The antibody also works well for immunohistochemistry and immunocytochemistry.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size ~ 32 kDa


Form Liquid
Purification Protein A purified
Buffer 100 mM Glycine (pH 8.0), 200 mM Tris
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 10215 Human OLIG2

GeneID: 50913 Mouse OLIG2

Swiss-port # Q13516 Human Oligodendrocyte transcription factor 2

Swiss-port # Q9EQW6 Mouse Oligodendrocyte transcription factor 2

Gene Symbol OLIG2
Gene Full Name oligodendrocyte transcription factor 2
Background Olig2 is a transcription factor which is required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain. Expressed in the ventral spinal cord as early as 9.5 dpc and scattered in the mantle zone, likely corresponding to oligodendrocyte progenitors migrating out from their site of origin. From 10.5 through 14.5 dpc, Olig2 is expressed in numerous cells in the ventricular and subventricular zones of the lateral and medial ganglionic eminences, suggesting that expression might not be limited to the oligodendrocytic lineage. Olig2 has further been shown to be critical in the proliferation of malignant glioma in brain tumors (Ligon et al., 2007).
Research Area Developmental Biology antibody; Gene Regulation antibody; Neuroscience antibody
Calculated MW 32 kDa

Images (2) Click the Picture to Zoom In

  • ARG52380 anti-Olig 2 antibody ICC/IF image

    Immunofluorescence: Primary rat cortical neuroepithelial cells stained with ARG52380 anti-Olig 2 antibody (red). The cells were treated with basic FGF for 30 hours prior to staining (this induces Olig 2 expression in these primary cells).

  • ARG52380 anti-Olig 2 antibody WB image

    Western blot: Neonatal rat brain lysate stained with ARG52380 anti-Olig 2 antibody.