anti-Olig 2 phospho (Ser10 / Ser13 / Ser14) antibody
anti-Olig 2 phospho (Ser10 / Ser13 / Ser14) antibody for Western blot and Rat
Developmental Biology antibody; Gene Regulation antibody; Neuroscience antibody
|Product Description||Rabbit Polyclonal antibody recognizes Olig 2 phospho (Ser10 / Ser13 / Ser14)|
|Predict Reactivity||Hu, Ms, Gpig, NHuPrm, Zfsh|
|Target Name||Olig 2|
|Immunogen||Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser10,13,14 conjugated to KLH|
|Alternate Names||bHLHb1; Class E basic helix-loop-helix protein 19; Class B basic helix-loop-helix protein 1; Oligo2; bHLHe19; Oligodendrocyte transcription factor 2; BHLHB1; Protein kinase C-binding protein 2; OLIGO2; Protein kinase C-binding protein RACK17; PRKCBP2; RACK17|
|Application Note||Specific for the ~32k Olig2 phosphorylated at Ser10, Ser13, and Ser14.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Buffer||10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol|
|Stabilizer||0.1 mg/ml BSA, 50% Glycerol|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||oligodendrocyte lineage transcription factor 2|
|Background||Olig2 is a well conserved bHLH transcription factor that shows both anti-neural functions and pro-neural functions at different stages in the formation of the oligodendrocyte lineage (Sun et al., 2011). Olig2 is expressed in 100% of the human diffuse gliomas irrespective of grade and required for intracranial tumor formation in a genetically relevant model of malignant glioma (Ligon et al., 2004; Ligon et al., 2007). A developmentally regulated triple serine motif at positions 10, 13 and 14 in the amino terminus is well conserved across species ranging from humans to zebrafish and is essential for Olig2 proliferative function in both normal and malignant neural progenitors (Sun et al., 2011). All three serine residues must be mutated to achieve a strong loss-of-function or gain-of-function phenotype, suggesting that the phosphorylation state of Olig2 represents a significant conformational change in the amino terminus (Sun et al., 2011).|
|Research Area||Developmental Biology antibody; Gene Regulation antibody; Neuroscience antibody|
|Calculated MW||32 kDa|
Images (1) Click the Picture to Zoom In
ARG52381 anti-Olig 2 phospho (Ser10 / Ser13 / Ser14) antibody WB image
Western blot: Rat neonatal brain lysate showing specific immunolabeling of the ~ 32k form of the Olig2 protein phosphorylated at Ser 10,13,14 stained with ARG52381 anti-Olig 2 phospho (Ser10 / Ser13 / Ser14) antibody.
Immunolabeling is blocked by the phospho-peptide used as antigen (peptide).