ARG54605

anti-Osteocalcin antibody [OC4-30]

anti-Osteocalcin antibody [OC4-30] for ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Western blot and Bovine,Chicken,Dog,Goat,Human,Mouse,Pig,Rabbit,Rat,Sheep

Developmental Biology antibody; Signaling Transduction antibody
publication_link Publication6

Overview

Product Description

Mouse Monoclonal antibody [OC4-30] recognizes Osteocalcin

Tested Reactivity Hu, Ms, Rat, Bov, Chk, Dog, Goat, Pig, Rb, Sheep
Tested Application ELISA, ICC/IF, IHC-Fr, IHC-P, WB
Specificity Includes the γ-carboxylated residue at position 17
Host Mouse
Clonality Monoclonal
Clone OC4-30
Isotype IgG2a
Target Name Osteocalcin
Antigen Species Bovine
Immunogen Bovine osteocalcin
Conjugation Un-conjugated
Alternate Names OCN; Gamma-carboxyglutamic acid-containing protein; Osteocalcin; OC; Bone Gla protein; BGP

Application Instructions

Application Note Sandwich ELISA: 1 - 10 ug/ml.
Western blot: 1 - 10 ug/ml, reducing or non-reducing conditions.
Immunohistochemistry: 1 - 10 ug/ml, paraffin-embedded or frozen tissue sections. Antigen retrieval: Proteinase K (0.4 mg/ml) at RT for 5 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer 10 mM PBS (pH 7.4) and 1% BSA
Stabilizer 1% BSA
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 12096 Mouse BGLAP

GeneID: 12097 Mouse BGLAP2

GeneID: 25295 Rat BGLAP

Gene Symbol BGLAP
Gene Full Name bone gamma-carboxyglutamate (gla) protein
Background This gene encodes a highly abundant bone protein secreted by osteoblasts that regulates bone remodeling and energy metabolism. The encoded protein contains a Gla (gamma carboxyglutamate) domain, which functions in binding to calcium and hydroxyapatite, the mineral component of bone. Serum osteocalcin levels may be negatively correlated with metabolic syndrome. Read-through transcription exists between this gene and the neighboring upstream gene, PMF1 (polyamine-modulated factor 1), but the encoded protein only shows sequence identity with the upstream gene product. [provided by RefSeq, Jun 2015]
Function Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. [UniProt]
Research Area Developmental Biology antibody; Signaling Transduction antibody
Calculated MW 11 kDa
PTM Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.

Images (2) Click the Picture to Zoom In

  • ARG54605 anti-Osteocalcin antibody [OC4-30] IHC-P image

    Immunohistochemistry: Parrafin-embedded chondroma tissue stained with ARG54605 anti-Osteocalcin antibody [OC4-30] at 1:200 (10 µg/ml) dilution, RT for 60 min. Antigen retrieval: Proteinase K (0.4 mg/ml) at RT for 5 min.

  • ARG54605 anti-Osteocalcin antibody [OC4-30] IHC-P image

    Immunohistochemistry: Parrafin-embedded synovial sarcoma tissue stained with ARG54605 anti-Osteocalcin antibody [OC4-30] at 1:200 (10 µg/ml) dilution, RT for 60 min. Antigen retrieval: Proteinase K (0.4 mg/ml) at RT for 5 min.

Specific References

Inhibiting PHD2 in human periodontal ligament cells via lentiviral vector-mediated RNA interference facilitates cell osteogenic differentiation and periodontal repair

WB / Human

Di Cui et al.
J Leukoc Biol.,  (2021)

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Simvastatin stimulates osteogenic differentiation of bone marrow mesenchymal stem cells.

ICC/IF / Mouse

Zhibin Guo et al.
Chinese Journal of Tissue Engineering Research.,  (2020)

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Peripheral sensory nerves regulate orthodontic tooth movement-induced bone formation via Wnt5a signaling pathway.

IHC-P / Rat

Lu Yeming et al.
新医学,  (2019)

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Identification of Gli1-interacting proteins during simvastatin-stimulated osteogenic differentiation of bone marrow mesenchymal stem cells.

WB, ICC/IF / Rat

Chi Bojing et al.
J Cell Biochem.,  (2019)

publication_link

 

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Stiffness Modification of Photopolymerizable Gelatin-Methacrylate Hydrogels Influences Endothelial Differentiation of Human Mesenchymal Stem Cells.

ICC/IF / Human

Lin Chih-Hsin et al.
J Tissue Eng Regen Med.,  (2018)

publication_link

 

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Inhibiting PHD2 in bone marrow mesenchymal stem cells via lentiviral vector-mediated RNA interference facilitates the repair of periodontal tissue defects in SD rats.

WB / Rat

Chen Chang-Xing et al.
Oncotarget.,  (2017)

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