ARG54607

anti-Osteocalcin antibody [OCG2]

anti-Osteocalcin antibody [OCG2] for ELISA,Immunohistochemistry,Western blot and Bovine,Human

Developmental Biology antibody; Signaling Transduction antibody

Overview

Product Description Mouse Monoclonal antibody [OCG2] recognizes Osteocalcin
Tested Reactivity Hu, Bov
Tested Application ELISA, IHC, WB
Host Mouse
Clonality Monoclonal
Clone OCG2
Isotype IgG3
Target Name Osteocalcin
Antigen Species Bovine
Immunogen Bovine osteocalcin
Epitope Residues 45-49
Conjugation Un-conjugated
Alternate Names OCN; Gamma-carboxyglutamic acid-containing protein; Osteocalcin; OC; Bone Gla protein; BGP

Application Instructions

Application Note Sandwich ELISA: 1 - 10 ug/ml.
Western blot: 1 - 10 ug/ml, reducing or non-reducing conditions.
Immunohistochemistry: 1 - 10 ug/ml, paraffin-embedded or frozen tissue sections.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer 10 mM PBS (pH 7.4) and 1% BSA
Stabilizer 1% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 281646 Bovine BGLAP

GeneID: 632 Human BGLAP

Swiss-port # P02818 Human Osteocalcin

Swiss-port # P02820 Bovine Osteocalcin

Gene Symbol BGLAP
Gene Full Name bone gamma-carboxyglutamate (gla) protein
Background This gene encodes a highly abundant bone protein secreted by osteoblasts that regulates bone remodeling and energy metabolism. The encoded protein contains a Gla (gamma carboxyglutamate) domain, which functions in binding to calcium and hydroxyapatite, the mineral component of bone. Serum osteocalcin levels may be negatively correlated with metabolic syndrome. Read-through transcription exists between this gene and the neighboring upstream gene, PMF1 (polyamine-modulated factor 1), but the encoded protein only shows sequence identity with the upstream gene product. [provided by RefSeq, Jun 2015]
Function Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. [UniProt]
Research Area Developmental Biology antibody; Signaling Transduction antibody
Calculated MW 11 kDa
PTM Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.

Clone References

Expansion and characterization of human embryonic stem cell-derived osteoblast-like cells.

IHC-P / Human

Arpornmaeklong P et al.
Cell Reprogram.,  (2010)

publication_link

 

hr_line

Phenotypic characterization, osteoblastic differentiation, and bone regeneration capacity of human embryonic stem cell-derived mesenchymal stem cells.

IHC-P / Human

Arpornmaeklong P et al.
Stem Cells Dev.,  (2009)

publication_link

 

hr_line