anti-Osteocalcin antibody [OCG2]
anti-Osteocalcin antibody [OCG2] for ELISA,Immunohistochemistry,Western blot and Bovine,Human
Developmental Biology antibody; Signaling Transduction antibody
|Product Description||Mouse Monoclonal antibody [OCG2] recognizes Osteocalcin|
|Tested Reactivity||Hu, Bov|
|Tested Application||ELISA, IHC, WB|
|Alternate Names||OCN; Gamma-carboxyglutamic acid-containing protein; Osteocalcin; OC; Bone Gla protein; BGP|
|Application Note||Sandwich ELISA: 1 - 10 ug/ml.
Western blot: 1 - 10 ug/ml, reducing or non-reducing conditions.
Immunohistochemistry: 1 - 10 ug/ml, paraffin-embedded or frozen tissue sections.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Buffer||10 mM PBS (pH 7.4) and 1% BSA|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||bone gamma-carboxyglutamate (gla) protein|
|Background||This gene encodes a highly abundant bone protein secreted by osteoblasts that regulates bone remodeling and energy metabolism. The encoded protein contains a Gla (gamma carboxyglutamate) domain, which functions in binding to calcium and hydroxyapatite, the mineral component of bone. Serum osteocalcin levels may be negatively correlated with metabolic syndrome. Read-through transcription exists between this gene and the neighboring upstream gene, PMF1 (polyamine-modulated factor 1), but the encoded protein only shows sequence identity with the upstream gene product. [provided by RefSeq, Jun 2015]|
|Function||Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. [UniProt]|
|Research Area||Developmental Biology antibody; Signaling Transduction antibody|
|Calculated MW||11 kDa|
|PTM||Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.|