ARG54606

anti-Osteocalcin antibody [OCG3]

anti-Osteocalcin antibody [OCG3] for ELISA,IHC-Frozen sections,Western blot and Bovine,Chicken,Dog,Goat,Human,Pig,Rabbit,Rat,Sheep

Developmental Biology antibody; Signaling Transduction antibody

Overview

Product Description

Mouse Monoclonal antibody [OCG3] recognizes Osteocalcin

Tested Reactivity Hu, Rat, Bov, Chk, Dog, Goat, Pig, Rb, Sheep
Tested Application ELISA, IHC-Fr, WB
Host Mouse
Clonality Monoclonal
Clone OCG3
Isotype IgG2
Target Name Osteocalcin
Antigen Species Bovine
Immunogen Bovine osteocalcin
Epitope Residues 21-31
Conjugation Un-conjugated
Alternate Names OCN; Gamma-carboxyglutamic acid-containing protein; Osteocalcin; OC; Bone Gla protein; BGP

Application Instructions

Application Note Sandwich ELISA: 1 - 10 ug/ml.
Western blot: 1 - 10 ug/ml, reducing or non-reducing conditions.
Immunohistochemistry: 1 - 10 ug/ml, paraffin-embedded (weak) or frozen tissue sections.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer 10 mM PBS (pH 7.4) and 1% BSA
Stabilizer 1% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 25295 Rat BGLAP

GeneID: 281646 Bovine BGLAP

GeneID: 396348 Chicken BGLAP

Gene Symbol BGLAP
Gene Full Name bone gamma-carboxyglutamate (gla) protein
Background This gene encodes a highly abundant bone protein secreted by osteoblasts that regulates bone remodeling and energy metabolism. The encoded protein contains a Gla (gamma carboxyglutamate) domain, which functions in binding to calcium and hydroxyapatite, the mineral component of bone. Serum osteocalcin levels may be negatively correlated with metabolic syndrome. Read-through transcription exists between this gene and the neighboring upstream gene, PMF1 (polyamine-modulated factor 1), but the encoded protein only shows sequence identity with the upstream gene product. [provided by RefSeq, Jun 2015]
Function Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium. [UniProt]
Research Area Developmental Biology antibody; Signaling Transduction antibody
Calculated MW 11 kDa
PTM Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.

Clone References

A tissue-engineered humanized xenograft model of human breast cancer metastasis to bone.

IHC-P / Human

Thibaudeau L et al.
Dis Model Mech.,  (2014)

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Calcium phosphate-bearing matrices induce osteogenic differentiation of stem cells through adenosine signaling.

Shih YR et al.
Proc Natl Acad Sci U S A.,  (2014)

publication_link

 

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Dynamics of bone healing after osteotomy with piezosurgery or conventional drilling - histomorphometrical, immunohistochemical, and molecular analysis.

IHC / Rat

Esteves JC et al.
J Transl Med.,  (2013)

publication_link

 

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Synthetic bone substitute engineered with amniotic epithelial cells enhances bone regeneration after maxillary sinus augmentation.

ICC/IF / Sheep

Barboni B et al.
PLoS One.,  (2013)

publication_link

 

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HIF-1α transgenic bone marrow cells can promote tissue repair in cases of corticosteroid-induced osteonecrosis of the femoral head in rabbits.

IHC-P / Rabbit

Ding H et al.
PLoS One.,  (2013)

publication_link

 

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MEPE-derived ASARM peptide inhibits odontogenic differentiation of dental pulp stem cells and impairs mineralization in tooth models of X-linked hypophosphatemia.

WB, IHC / Human

Salmon B et al.
PLoS One.,  (2013)

publication_link

 

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Adhesion, vitality and osteogenic differentiation capacity of adipose derived stem cells seeded on nitinol nanoparticle coatings.

ICC/IF / Human

Strauss S et al.
PLoS One.,  (2013)

publication_link

 

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Induction of osteogenic differentiation of adipose derived stem cells by microstructured nitinol actuator-mediated mechanical stress.

ICC/IF / Human

Strauß S et al.
PLoS One.,  (2012)

publication_link

 

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Heparan sulfate enhances the self-renewal and therapeutic potential of mesenchymal stem cells from human adult bone marrow.

IHC-P / Rat

Helledie T et al.
Stem Cells Dev.,  (2012)

publication_link

 

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Expression of chondro-osteogenic BMPs in clinical samples of patellar tendinopathy.

IHC-P / Human

Rui YF et al.
Knee Surg Sports Traumatol Arthrosc.,  (2012)

publication_link

 

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Inhibition of PHOSPHO1 activity results in impaired skeletal mineralization during limb development of the chick.

WB / Chicken

Macrae VE et al.
Bone.,  (2010)

publication_link

 

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