anti-PARK7 / DJ1 antibody [4H4]

anti-PARK7 / DJ1 antibody [4H4] for ICC/IF,Western blot and Human,Bovine

Cancer antibody; Gene Regulation antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody


Product Description Mouse Monoclonal antibody [4H4] recognizes PARK7 / DJ1
Tested Reactivity Hu, Bov
Species Does Not React With Ms, Rat
Tested Application ICC/IF, WB
Host Mouse
Clonality Monoclonal
Clone 4H4
Isotype IgG1
Target Name PARK7 / DJ1
Antigen Species Human
Immunogen Full length recombinant human PARK7 / DJ-1 expressed in and purified from E. coli
Conjugation Un-conjugated
Alternate Names DJ1; DJ-1; Oncogene DJ1; EC 3.5.1.-; Parkinson disease protein 7; HEL-S-67p; EC 3.1.2.-; Protein deglycase DJ-1

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:2000 - 1:5000
Application Note Specific for the ~21k park7 protein.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Affinity Purified
Buffer PBS and 10 mM Sodium azide
Preservative 10 mM Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 11315 Human PARK7

GeneID: 511268 Bovine PARK7

Swiss-port # Q5E946 Bovine Protein deglycase DJ-1

Swiss-port # Q99497 Human Protein deglycase DJ-1

Gene Symbol PARK7
Gene Full Name parkinson protein 7
Background Park7, also known as DJ-1, is a member of the peptidase C56 family of proteins and is thought to function as a molecular chaperone. Mutations in park7 have been associated with autosomal recessive, early onset Parkinson’s disease (Bonifati et al., 2003). Recently, park7 has been shown to inhibit microtubule associated protein 1B aggregation thus leading to neuronal apoptosis (Wang et al., 2011).
Highlight Related products:
PARK7 antibodies; Anti-Mouse IgG secondary antibodies;
Related news:
Astrocyte-to-neuron conversion for Parkinson's disease treatment
Research Area Cancer antibody; Gene Regulation antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Calculated MW 20 kDa
PTM Sumoylated on Lys-130 by PIAS2 or PIAS4; which is enhanced after ultraviolet irradiation and essential for cell-growth promoting activity and transforming activity.
Cys-106 is easily oxidized to sulfinic acid.
Undergoes cleavage of a C-terminal peptide and subsequent activation of protease activity in response to oxidative stress.

Images (2) Click the Picture to Zoom In

  • ARG52388 anti-PARK7 / DJ1 antibody [4H4] ICC/IF image

    Immunofluorescence: HeLa cells stained with ARG52388 anti-PARK7 / DJ1 antibody [4H4] (green) showing strong cytoplasmic staining and ARG52468 anti-Vimentin antibody (red).

  • ARG52388 anti-PARK7 / DJ1 antibody [4H4] WB image

    Western blot: Rat brain, Mouse brain, NIH/3T3, HeLa, HEK293 and C6 cell lysates stained with ARG52388 anti-PARK7 / DJ1 antibody [4H4] (green) at 1:5000 dilution. The blot was simultaneously stained with ARG52468 anti-Vimentin antibody (red) at 1:5000 dilution.

    Clone 4H4 detects protein with apparent molecular weight of 21 kDa but only in Human cell lines, since it does not recognize the Mouse or Rat DJ1 protein.