anti-PARP (full length) antibody

anti-PARP (full length) antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody; Apoptosis Marker antibody; Mitochondria/Caspase Dependant Apoptosis Marker antibody


Product Description Rabbit Polyclonal antibody recognizes PARP (full length)
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Specificity This antibody majorly reacts to full length of PARP protein and may reacts to 89kda cleaved PARP fragment with weak signal.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PARP (full length)
Antigen Species Human
Immunogen Recombinant protein of Human PARP.
Conjugation Un-conjugated
Alternate Names EC; Poly[ADP-ribose] synthase 1; PPOL; ADPRT; ARTD1; NAD; PARP-1; ADPRT 1; Poly [ADP-ribose] polymerase 1; PARP; ADP-ribosyltransferase diphtheria toxin-like 1; ADPRT1; pADPRT-1

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HepG2 and SKOV3
Observed Size ~ 115 kDa


Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 11545 Mouse PARP1

GeneID: 142 Human PARP1

GeneID: 25591 Rat PARP1

Gene Symbol PARP1
Gene Full Name poly (ADP-ribose) polymerase 1
Background This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]
Function Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Required for PARP9 and DTX3L recruitment to DNA damage sites. PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites. [UniProt]
Cellular Localization Nucleus, nucleolus. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Gene Regulation antibody; Metabolism antibody; Apoptosis Marker antibody; Mitochondria/Caspase Dependant Apoptosis Marker antibody
Calculated MW 113 kDa
PTM Phosphorylated by PRKDC and TXK.

Poly-ADP-ribosylated by PARP2; poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites (PubMed:19661379). ADP-ribosylated on serine by autocatalysis; serine ADP-ribosylation takes place following interaction with HPF1 (PubMed:28190768).

S-nitrosylated, leading to inhibit transcription regulation activity. [UniProt]

Images (3) Click the Picture to Zoom In

  • ARG57713 anti-PARP (full length) antibody WB image

    Western blot: 25 µg of HepG2 and SKOV3 cell lysates stained with ARG57713 anti-PARP (full length) antibody at 1:1000 dilution.

  • ARG57713 anti-PARP (full length) antibody ICC/IF image

    Immunofluorescence: U2OS cells stained with ARG57713 anti-PARP (full length) antibody at 1:100 dilution.

  • ARG57713 anti-PARP (full length) antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat testis stained with ARG57713 anti-PARP (full length) antibody at 1:100 dilution (40x lens).