anti-PARP2 antibody

anti-PARP2 antibody for Flow cytometry,Western blot and Human,Mouse,Rat


Product Description Rabbit Polyclonal antibody recognizes PARP2
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PARP2
Antigen Species Human
Immunogen Synthetic peptide derived from Human PARP2.
Conjugation Un-conjugated
Alternate Names EC; hPARP-2; ARTD2; NAD; pADPRT-2; PARP-2; Poly [ADP-ribose] polymerase 2; Poly[ADP-ribose] synthase 2; ADP-ribosyltransferase diphtheria toxin-like 2; ADPRT-2; ADPRTL2; ADPRTL3; ADPRT2

Application Instructions

Application Suggestion
Tested Application Dilution
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Raji
Observed Size ~ 65 kDa


Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 10038 Human PARP2

GeneID: 11546 Mouse PARP2

Swiss-port # O88554 Mouse Poly [ADP-ribose] polymerase 2

Swiss-port # Q9UGN5 Human Poly [ADP-ribose] polymerase 2

Gene Symbol PARP2
Gene Full Name poly (ADP-ribose) polymerase 2
Background This gene encodes poly(ADP-ribosyl)transferase-like 2 protein, which contains a catalytic domain and is capable of catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. The basic residues within the N-terminal region of this protein may bear potential DNA-binding properties, and may be involved in the nuclear and/or nucleolar targeting of the protein. Two alternatively spliced transcript variants encoding distinct isoforms have been found. [provided by RefSeq, Jul 2008]
Function Poly-ADP-ribosyltransferase that mediates poly-ADP-ribosylation of proteins and plays a key role in DNA repair (PubMed:10364231, PubMed:28190768, PubMed:25043379). Mainly mediates glutamate and aspartate ADP-ribosylation of target proteins: the ADP-D-ribosyl group of NAD(+) is transferred to the acceptor carboxyl group of glutamate and aspartate residues and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units (PubMed:25043379). ADP-ribosylation follows DNA damage and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks (PubMed:10364231). Also mediates serine ADP-ribosylation of target proteins following interaction with HPF1; HPF1 conferring serine specificity (PubMed:28190768). In addition to proteins, also able to ADP-ribosylate DNA: preferentially acts on 5'-terminal phosphates at DNA strand breaks termini in nicked duplex (PubMed:27471034). [UniProt]
Cellular Localization Nucleus. [UniProt]
Calculated MW 66 kDa
PTM Poly-ADP-ribosylated by PARP1.

Acetylation reduces DNA binding and enzymatic activity. [UniProt]

Images (1) Click the Picture to Zoom In

  • ARG42604 anti-PARP2 antibody WB image

    Western blot: Raji cell lysate stained with ARG42604 anti-PARP2 antibody.