ARG66582
anti-PCCB antibody
anti-PCCB antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes PCCB |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PCCB |
| Antigen Species | Human |
| Immunogen | Recombinant full length protein of Human PCCB. |
| Conjugation | Un-conjugated |
| Alternate Names | PCCase subunit beta; EC 6.4.1.3; Propanoyl-CoA:carbon dioxide ligase subunit beta; Propionyl-CoA carboxylase beta chain, mitochondrial |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.42% Potassium phosphate (pH 7.3), 0.87% NaCl, 0.01% Sodium azide and 30% Glycerol. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 30% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P05166 Human Propionyl-CoA carboxylase beta chain, mitochondrial Swiss-port # Q99MN9 Mouse Propionyl-CoA carboxylase beta chain, mitochondrial |
|---|---|
| Gene Symbol | PCCB |
| Gene Full Name | propionyl CoA carboxylase, beta polypeptide |
| Background | The protein encoded by this gene is a subunit of the propionyl-CoA carboxylase (PCC) enzyme, which is involved in the catabolism of propionyl-CoA. PCC is a mitochondrial enzyme that probably acts as a dodecamer of six alpha subunits and six beta subunits. This gene encodes the beta subunit of PCC. Defects in this gene are a cause of propionic acidemia type II (PA-2). Multiple transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, May 2010] |
| Cellular Localization | Mitochondrion matrix. [UniProt] |
| Calculated MW | 58 kDa |
Images (3) Click the Picture to Zoom In
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ARG66582 anti-PCCB antibody ICC/IF image
Immunofluorescence: Formalin-fixed U2OS cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were stained with ARG66582 anti-PCCB antibody (red) in 3% BSA-PBS and incubated overnight at 4°C.
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ARG66582 anti-PCCB antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human kidney tissue. Antigen Retrieval: Heat mediation was performed in Sodium citrate buffer (pH 6.0). The section was then stained with ARG66582 anti-PCCB antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was counterstained with haematoxylin and mounted with DPX.
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ARG66582 anti-PCCB antibody WB image
Western blot: HT29, Mouse liver and Mouse heart lysates stained with ARG66582 anti-PCCB antibody.
