anti-PCNA antibody [PC10]

anti-PCNA antibody [PC10] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,IHC-Frozen sections,Immunoprecipitation,Western blot and Chicken,D. melanogaster ,Human,Monkey,Mouse,Pig,Pigeon,Rat,Zebrafish

Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Gene Regulation antibody
publication_link Publication9


Product Description

Mouse Monoclonal antibody [PC10] recognizes PCNA

Tested Reactivity Hu, Ms, Rat, Chk, Dm, Mk, Pgn, Pig, Zfsh
Tested Application FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB
Host Mouse
Clonality Monoclonal
Clone PC10
Isotype IgG2a
Target Name PCNA
Antigen Species Rat
Immunogen Protein A-rat PCNA (proliferating cell nuclear antigen) fusion obtained from pC2T
Conjugation Un-conjugated
Alternate Names PCNA; ATLD2; Cyclin; Proliferating cell nuclear antigen

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1 µg/10^6 cells
ICC/IF0.5 - 1 µg/ml.
IHC-P0.5 - 1 µg/ml
WB0.01 - 1 µg/ml
Application Note ICC/IF: Methanol fixed samples.
IHC-P: Antigen Retrieval: Heat mediated in Citrate buffer (pH 6.0)
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HEK293


Form Liquid
Buffer PBS (pH 7.6), 0.02% Sodium azide and 0.4M arginine.
Preservative 0.02% Sodium azide
Concentration 0.2 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 18538 Mouse PCNA

GeneID: 25737 Rat PCNA

GeneID: 30678 Zebrafish PCNA

Gene Symbol Pcna
Gene Full Name proliferating cell nuclear antigen
Background The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]
Function Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion (By similarity). [UniProt]
Cellular Localization Nucleus. Note: Forms nuclear foci representing sites of ongoing DNA replication and vary in morphology and number during S phase. Together with APEX2, is redistributed in discrete nuclear foci in presence of oxidative DNA damaging agents. [uniprot: P12004]
Highlight Related products:
PCNA antibodies; PCNA Duos / Panels; Anti-Mouse IgG secondary antibodies;
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Gene Regulation antibody
Calculated MW 29 kDa
PTM Phosphorylated. Phosphorylation at Tyr-211 by EGFR stabilizes chromatin-associated PCNA.
Acetylated by CREBBP and p300/EP300; preferentially acetylated by CREBBP on Lys-80, Lys-13 and Lys-14 and on Lys-77 by p300/EP300 upon loading on chromatin in response to UV irradiation (PubMed:24939902, PubMed:19419956). Lysine acetylation disrupts association with chromatin, hence promoting PCNA ubiquitination and proteasomal degradation in response to UV damage in a CREBBP- and EP300-dependent manner (PubMed:24939902). Acetylation disrupts interaction with NUDT15 and promotes degradation (PubMed:19419956).
Ubiquitinated (PubMed:24939902, PubMed:20227374). Following DNA damage, can be either monoubiquitinated to stimulate direct bypass of DNA lesions by specialized DNA polymerases or polyubiquitinated to promote recombination-dependent DNA synthesis across DNA lesions by template switching mechanisms. Following induction of replication stress, monoubiquitinated by the UBE2B-RAD18 complex on Lys-164, leading to recruit translesion (TLS) polymerases, which are able to synthesize across DNA lesions in a potentially error-prone manner. An error-free pathway also exists and requires non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH. This error-free pathway, also known as template switching, employs recombination mechanisms to synthesize across the lesion, using as a template the undamaged, newly synthesized strand of the sister chromatid. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis. Sumoylated during S phase.
Methylated on glutamate residues by ARMT1/C6orf211.

Images (2) Click the Picture to Zoom In

  • ARG62605 anti-PCNA antibody [PC10] WB image

    Western blot: A549, H1299, HCT116 and HepG2 cell lysates stained with ARG62605 anti-PCNA antibody [PC10] at 0.2 µg/ml.

  • ARG62605 anti-PCNA antibody [PC10] IHC-P image

    Immunohistochemistry: formalin-fixed, paraffin-embedded human breast carcinoma tissue stained with PCNA antibody [PC10] (ARG62605).

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Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC

Specific References

E2F1/CKS2/PTEN signaling axis regulates malignant phenotypes in pediatric retinoblastoma

WB / Human

Meng Chen et al.
Cell Death Dis,  (2022)




Downregulation of AKT/mTOR signaling pathway for Salmonella-mediated autophagy in human anaplastic thyroid cancer

IHC-P / Mouse

Li-Hsien Wu et al.
J Cancer,  (2022)




Mesenchymal Stem Cells-Derived Exosomes Ameliorate Ischemia/Reperfusion Induced Acute Kidney Injury in a Porcine Model

WB / Pig

Jianni Huang et al.
Frontiers in Cell and Developmental Biology,  (2022)




Single-cell analyses unravel cell type-specific responses to a vitamin D analog in prostatic precancerous lesions.

IHC-P / Mouse

Mohamed A Abu El Maaty et al.
Sci Adv.,  (2021)




Metformin as a senostatic drug enhances the anticancer efficacy of CDK4/6 inhibitor in head and neck squamous cell carcinoma.

IHC-P / Human

Qinchao Hu et al.
Cell Death Dis.,  (2020)




Discoidin domain receptor 1 regulates endochondral ossification through terminal differentiation of chondrocytes.

IHC-P / Mouse

Chou Liang-Yin et al.
FASEB J.,  (2020)




Protein arginine methyltransferase 1 mediates renal fibroblast activation and fibrogenesis through activation of Smad3 signaling.

IHC-P / Rat

Zhu Yu et al.
Am J Physiol Renal Physiol.,  (2020)




CSN6 expression is associated with pancreatic cancer progression and predicts poor prognosis.

WB, IHC-P / Human

Shi Jiaqi et al.
Cancer Biol Ther.,  (2019)




STAT1-mediated inhibition of FOXM1 enhances gemcitabine sensitivity in pancreatic cancer.

IHC-P / Human

Liu Chao et al.
Clin Sci (Lond).,  (2019)