anti-PCNA antibody [SQab18115]
anti-PCNA antibody [SQab18115] for Western blot,Flow cytometry,Immunoprecipitation and Human,Mouse,Rat,Bovine,Dog,Chicken,African green monkey
|Product Description||Recombinant Rabbit Monoclonal antibody [SQab18115] recognizes PCNA|
|Tested Reactivity||Hu, Ms, Rat, AGMK, Bov, Chk, Dog|
|Tested Application||FACS, IP, WB|
|Immunogen||Synthetic peptide corresponding to aa. 100-200 of Human PCNA.|
|Alternate Names||PCNA; ATLD2; Cyclin; Proliferating cell nuclear antigen|
|Application Note||* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.|
|Purification||Purification with Protein A.|
|Buffer||PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.|
|Preservative||0.01% Sodium azide|
|Stabilizer||40% Glycerol and 0.05% BSA|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||proliferating cell nuclear antigen|
|Background||The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]|
|Function||Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'-5' exonuclease and 3'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways. Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion. [UniProt]|
|Calculated MW||29 kDa|
|PTM||Phosphorylated. Phosphorylation at Tyr-211 by EGFR stabilizes chromatin-associated PCNA.
Acetylated by CREBBP and p300/EP300; preferentially acetylated by CREBBP on Lys-80, Lys-13 and Lys-14 and on Lys-77 by p300/EP300 upon loading on chromatin in response to UV irradiation (PubMed:24939902, PubMed:19419956). Lysine acetylation disrupts association with chromatin, hence promoting PCNA ubiquitination and proteasomal degradation in response to UV damage in a CREBBP- and EP300-dependent manner (PubMed:24939902). Acetylation disrupts interaction with NUDT15 and promotes degradation (PubMed:19419956).
Ubiquitinated (PubMed:24939902, PubMed:20227374). Following DNA damage, can be either monoubiquitinated to stimulate direct bypass of DNA lesions by specialized DNA polymerases or polyubiquitinated to promote recombination-dependent DNA synthesis across DNA lesions by template switching mechanisms. Following induction of replication stress, monoubiquitinated by the UBE2B-RAD18 complex on Lys-164, leading to recruit translesion (TLS) polymerases, which are able to synthesize across DNA lesions in a potentially error-prone manner. An error-free pathway also exists and requires non-canonical polyubiquitination on Lys-164 through 'Lys-63' linkage of ubiquitin moieties by the E2 complex UBE2N-UBE2V2 and the E3 ligases, HLTF, RNF8 and SHPRH. This error-free pathway, also known as template switching, employs recombination mechanisms to synthesize across the lesion, using as a template the undamaged, newly synthesized strand of the sister chromatid. Monoubiquitination at Lys-164 also takes place in undamaged proliferating cells, and is mediated by the DCX(DTL) complex, leading to enhance PCNA-dependent translesion DNA synthesis. Sumoylated during S phase.
Methylated on glutamate residues by ARMT1/C6orf211. [UniProt]
Images (4) Click the Picture to Zoom In
ARG66388 anti-PCNA antibody [SQab18115] FACS image
Flow Cytometry: HeLa cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were then stained with ARG66388 anti-PCNA antibody [SQab18115] (red) at 1:2000 dilution in 1x PBS/1% BSA for 30 min at room temperature, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control.
ARG66388 anti-PCNA antibody [SQab18115] WB image
Western blot: 10 µg of A431, 293, HeLa, HepG2, MCF7, 3T3 and PC-12 cell lysates stained with ARG66388 anti-PCNA antibody [SQab18115] at 1:2000 dilution.
ARG66388 anti-PCNA antibody [SQab18115] IP image
Immunoprecipitation: 0.4 mg of HeLa whole cell lysate was immunoprecipitated (1:50 dilution) and stained with ARG66388 anti-PCNA antibody [SQab18115].
Lane 1: Immunoprecipitation in HeLa whole cell lysate
Lane 2: PBS instead of Primary Ab in HeLa whole cell lysate
Lane 3: HeLa whole cell lysate, 10 µg (input)
ARG66388 anti-PCNA antibody [SQab18115] WB image
Western blot: 10 µg of MDCK, MDBK, COS-7 and Chicken heart lysates stained with ARG66388 anti-PCNA antibody [SQab18115] at 1:2000 dilution.