ARG10790

anti-PDE12 antibody

anti-PDE12 antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes PDE12
Tested Reactivity Hu, Ms, Rat
Tested Application ELISA, IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PDE12
Antigen Species Rat
Immunogen Synthetic peptide taken within aa. 370-420 from Rat PDE12.
Conjugation Un-conjugated
Full Name phosphodiesterase 12
Alternate Names EC 3.1.4.-; 2',5'-phosphodiesterase 12; Mitochondrial deadenylase; EC 3.1.13.4; 2'-PDE; 2-PDE

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA1:10000
IHC-P1:50 - 1:100
IP1:200
WB1:500
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 67 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer Tris-Glycine Buffer (pH 7.4 - 7.8), Hepes, 0.02% Sodium azide, 30% Glycerol and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 30% Glycerol and 0.5% BSA
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 201626 Human PDE12

GeneID: 211948 Mouse PDE12

Swiss-port # Q3TIU4 Mouse 2',5'-phosphodiesterase 12

Swiss-port # Q6L8Q7 Human 2',5'-phosphodiesterase 12

Gene Symbol Pde12
Gene Full Name phosphodiesterase 12
Function Enzyme that cleaves 2',5'-phosphodiester bond linking adenosines of the 5'-triphosphorylated oligoadenylates, triphosphorylated oligoadenylates referred as 2-5A modulates the 2-5A system. This enzyme degraded triphosphorylated 2-5A to produce AMP and ATP. Also cleaves 3',5'-phosphodiester bond of oligoadenylates. Plays a role as a negative regulator of the The 2-5A system that is one of the major pathways for antiviral and antitumor functions induced by interferons (IFNs). Suppression of this enzyme induces reduction of viral replication in Hela cells, thus counteracting the antiviral pathway probably by inhibiting the 2-5A system. [UniProt]

Images (3) Click the Picture to Zoom In

  • ARG10790 anti-PDE12 antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Mouse kidney stained with ARG10790 anti-PDE12 antibody at 1:100 dilution.

  • ARG10790 anti-PDE12 antibody WB image

    Western blot: 1) Rat kidney, 2) Rat spleen, and 3) Rat brain lysates stained with ARG10790 anti-PDE12 antibody at 1:500 dilution.

  • ARG10790 anti-PDE12 antibody IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded Mouse kidney stained with ARG10790 anti-PDE12 antibody at 1:100 dilution.