ARG10796

anti-PDE3A antibody

anti-PDE3A antibody for Confocal microscopy (Confocal),Dot blot,ELISA,Fluorescence Resonance Energy Transfer,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Monkey,Mouse,Rat,Xenopus

publication_link Publication1

Overview

Product Description Rabbit Polyclonal antibody recognizes PDE3A
Tested Reactivity Hu, Ms, Rat, Mk, Xenopus
Tested Application Confocal, Dot, ELISA, FRET, ICC/IF, IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PDE3A
Antigen Species Human
Immunogen Synthetic peptide selective only for PDE3A variants.
Conjugation Un-conjugated
Full Name phosphodiesterase 3A, cGMP-inhibited
Alternate Names EC 3.1.4.17; Cyclic GMP-inhibited phosphodiesterase A; CGI-PDE; CGI-PDE A; CGI-PDE-A; HTNB; cGMP-inhibited 3',5'-cyclic phosphodiesterase A

Application Instructions

Application Suggestion
Tested Application Dilution
Confocal1:250
Dot1:10000
ELISA1:10000
FRETAssay-dependent
ICC/IF1:250
IHC-P1:250
IP1:200
WB1:500
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 125 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer Tris-Glycine Buffer (pH 7.4 - 7.8), Hepes, 0.02% Sodium azide, 30% Glycerol and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 30% Glycerol and 0.5% BSA
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 50678 Rat PDE3A

GeneID: 5139 Human PDE3A

GeneID: 54611 Mouse PDE3A

Gene Symbol PDE3A
Gene Full Name phosphodiesterase 3A, cGMP-inhibited
Background This gene encodes a member of the cGMP-inhibited cyclic nucleotide phosphodiesterase (cGI-PDE) family. cGI-PDE enzymes hydrolyze both cAMP and cGMP, and play critical roles in many cellular processes by regulating the amplitude and duration of intracellular cyclic nucleotide signals. The encoded protein mediates platelet aggregation and also plays important roles in cardiovascular function by regulating vascular smooth muscle contraction and relaxation. Inhibitors of the encoded protein may be effective in treating congestive heart failure. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Sep 2011]
Function Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. [UniProt]

Images (2) Click the Picture to Zoom In

  • ARG10796 anti-PDE3A antibody ICC/IF image

    Immunofluorescence: Ice-cold Acetone/Methanol (1:1) fixed (5 min) cultured rat smooth muscle cells stained with ARG10796 anti-PDE3A antibody (red) at 1:100 dilution. Nuclei were counterstained with DAPI (blue).

  • ARG10796 anti-PDE3A antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human ovarian medulla tissue stained with ARG10796 anti-PDE3A antibody at 1:100 dilution.

Specific References

Electroacupuncture promotes the gastrointestinal motility of diabetic mice by CNP/NPR-B-cGMP and PDE3A-cGMP signaling.

WB / Mouse

An Jing et al.
Neurogastroenterol Motil.,  (2019)

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