ARG10819

anti-PDE4D phospho (Ser190) antibody

anti-PDE4D phospho (Ser190) antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot,Dot blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes PDE4D phospho (Ser190)
Tested Reactivity Hu, Ms, Rat
Tested Application Dot, ELISA, IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PDE4D
Species Human
Immunogen Synthetic peptide selective for PDE4D PKA site taken within aa. 50-100 from Human PDE4D. Derived around the PKA phosphorylation site of Ser190: R-E-Sp-F-L.
Conjugation Un-conjugated
Full Name phosphodiesterase 4D, cAMP-specific
Alternate Names EC 3.1.4.53; STRK1; DPDE3; PDE43; ACRDYS2; PDE4DN2; cAMP-specific 3',5'-cyclic phosphodiesterase 4D; HSPDE4D

Application Instructions

Application Suggestion
Tested Application Dilution
Dot1:10000
ELISA1:10000
IHC-P1:50 - 1:100
IP1:150
WB1:500
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 91 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer Tris-Glycine Buffer (pH 7.4 - 7.8), Hepes, 0.02% Sodium azide, 30% Glycerol and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 30% Glycerol and 0.5% BSA
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 238871 Mouse PDE4D

GeneID: 24627 Rat PDE4D

GeneID: 5144 Human PDE4D

Gene Symbol PDE4D
Gene Full Name phosphodiesterase 4D, cAMP-specific
Background This gene encodes one of four mammalian counterparts to the fruit fly 'dunce' gene. The encoded protein has 3',5'-cyclic-AMP phosphodiesterase activity and degrades cAMP, which acts as a signal transduction molecule in multiple cell types. This gene uses different promoters to generate multiple alternatively spliced transcript variants that encode functional proteins.[provided by RefSeq, Sep 2009]
Function Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. [UniProt]
PTM Long isoforms that share a conserved PKA phosphorylation site in the N-terminus are activated by PKA through phosphorylation (By similarity). Isoform 3 and isoform 7 are activated by phosphorylation (in vitro), but not isoform 6. Isoform N3 and isoform 12 are phosphorylated on Ser-49, Ser-51, Ser-55 and Ser-59.
Sumoylation of long isoforms by PIAS4 augments their activation by PKA phosphorylation and represses their inhibition by ERK phosphorylation.

Images (1) Click the Picture to Zoom In

  • ARG10819 anti-PDE4D phospho (Ser190) antibody WB image

    Western blot: Non-phosphorylated and phosphorylated PDE4D protein stained with ARG10819 anti-PDE4D phospho (Ser190) antibody and ARG10803 anti-PDE4D antibody at 1:500 dilution.