ARG10809

anti-PDE8A antibody

anti-PDE8A antibody for Confocal microscopy (Confocal),ELISA,ICC/IF,Immunohistochemistry,Immunoprecipitation,Western blot,Dot blot and Bovine,Human,Monkey,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes PDE8A
Tested Reactivity Hu, Ms, Rat, Bov, Mk
Tested Application Confocal, Dot, ELISA, ICC/IF, IHC, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PDE8A
Antigen Species Human
Immunogen Synthetic cyclic peptide from C-terminus common to all PDE8A variants.
Conjugation Un-conjugated
Full Name phosphodiesterase 8A
Alternate Names HsT19550; High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A; EC 3.1.4.53

Application Instructions

Application Suggestion
Tested Application Dilution
Confocal1:250
Dot1:10000
ELISA1:10000
ICC/IF1:250
IHC1:250
IP1:250
WB1:500
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 93 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer Tris-Glycine Buffer (pH 7.4 - 7.8), Hepes, 0.02% Sodium azide, 30% Glycerol and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 30% Glycerol and 0.5% BSA
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 18584 Mouse PDE8A

GeneID: 5151 Human PDE8A

Swiss-port # O60658 Human High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiest

Swiss-port # O88502 Mouse High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiest

Gene Symbol PDE8A
Gene Full Name phosphodiesterase 8A
Background The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE8 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]
Function Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in maintaining basal levels of the cyclic nucleotide and/or in the cAMP regulation of germ cell development. [UniProt]
PTM Phosphorylated at Ser-359 by PKA under elevated cAMP conditions, this enhances catalytic activity.

Images (1) Click the Picture to Zoom In

  • ARG10809 anti-PDE8A antibody WB image

    Western blot: Cytosolic PDE8A samples stained with ARG10809 anti-PDE8A antibody at 1:500 dilution.