ARG10811

anti-PDE8B antibody

anti-PDE8B antibody for Confocal microscopy (Confocal),ELISA,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot,Dot blot and Bovine,Human,Monkey,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes PDE8B
Tested Reactivity Hu, Ms, Rat, Bov, Mk
Tested Application Confocal, Dot, ELISA, ICC/IF, IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PDE8B
Antigen Species Mouse
Immunogen Synthetic cyclic peptide common to all PDE8B variants.
Conjugation Un-conjugated
Full Name phosphodiesterase 8B
Alternate Names EC 3.1.4.53; HsPDE8B; Cell proliferation-inducing gene 22 protein; ADSD; PPNAD3; High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B

Application Instructions

Application Suggestion
Tested Application Dilution
Confocal1:200
Dot1:10000
ELISA1:10000
ICC/IF1:200
IHC-P1:200
IP1:250
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 99 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer Tris-Glycine Buffer (pH 7.4 - 7.8), Hepes, 0.02% Sodium azide, 30% Glycerol and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 30% Glycerol and 0.5% BSA
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 8622 Human PDE8B

Swiss-port # O95263 Human High affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiest

Gene Symbol Pde8b
Gene Full Name phosphodiesterase 8B
Background The protein encoded by this gene is a cyclic nucleotide phosphodiesterase (PDE) that catalyzes the hydrolysis of the second messenger cAMP. The encoded protein, which does not hydrolyze cGMP, is resistant to several PDE inhibitors. Defects in this gene are a cause of autosomal dominant striatal degeneration (ADSD). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2010]
Function Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in specific signaling in the thyroid gland. [UniProt]

Images (2) Click the Picture to Zoom In

  • ARG10811 anti-PDE8B antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human ovarian medulla tissue stained with ARG10811 anti-PDE8B antibody.

  • ARG10811 anti-PDE8B antibody WB image

    Western blot: Rat testis and Rat heart lysates stained with ARG10811 anti-PDE8B antibody at 1:300 dilution.