ARG10812

anti-PDE9A antibody

anti-PDE9A antibody for Confocal microscopy (Confocal),ELISA,ICC/IF,Immunohistochemistry,Immunoprecipitation,Western blot,Dot blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes PDE9A
Tested Reactivity Hu, Ms, Rat
Tested Application Confocal, Dot, ELISA, ICC/IF, IHC, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PDE9A
Antigen Species Human
Immunogen Synthetic cyclic peptide common to all PDE9A variants.
Conjugation Un-conjugated
Full Name phosphodiesterase 9A
Alternate Names High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A; EC 3.1.4.35; HSPDE9A2

Application Instructions

Application Suggestion
Tested Application Dilution
Confocal1:100
Dot1:100
ELISA1:100
ICC/IF1:100
IHC1:100
IP1:250
WB1:500
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 68 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer Tris-Glycine Buffer (pH 7.4 - 7.8), Hepes, 0.02% Sodium azide, 30% Glycerol and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 30% Glycerol and 0.5% BSA
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 18585 Mouse PDE9A

GeneID: 191569 Rat PDE9A

GeneID: 5152 Human PDE9A

Gene Symbol PDE9A
Gene Full Name phosphodiesterase 9A
Background The protein encoded by this gene catalyzes the hydrolysis of cAMP and cGMP to their corresponding monophosphates. The encoded protein plays a role in signal transduction by regulating the intracellular concentration of these cyclic nucleotides. Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
Function Specifically hydrolyzes the second messenger cGMP, which is a key regulator of many important physiological processes. Highly specific: compared to other members of the cyclic nucleotide phosphodiesterase family, has the highest affinity and selectivity for cGMP. Specifically regulates natriuretic-peptide-dependent cGMP signaling in heart, acting as a regulator of cardiac hypertrophy in myocytes and muscle. Does not regulate nitric oxide-dependent cGMP in heart. Additional experiments are required to confirm whether its ability to hydrolyze natriuretic-peptide-dependent cGMP is specific to heart or is a general feature of the protein (Probable). In brain, involved in cognitive function, such as learning and long-term memory (By similarity). [UniProt]

Images (2) Click the Picture to Zoom In

  • ARG10812 anti-PDE9A antibody WB image

    Western blot: Recombinant PDE9A protein stained with ARG10812 anti-PDE9A antibody at 1:500 dilution.

  • ARG10812 anti-PDE9A antibody WB image

    Western blot: PDE9A on SDS-PAGE showing tissue distribution of PDE9A. The blots were stained with ARG10812 anti-PDE9A antibody at 1:1000 dilution.