ARG22292

anti-PDI antibody

anti-PDI antibody for Western blot,Immunohistochemistry,ICC/IF,Immunoprecipitation and Dog,Human,Monkey,Mouse,Rat,Xenopus laevis,Bovine,Guinea pig,Hamster,Pig,Sheep

Overview

Product Description Rabbit Polyclonal antibody recognizes PDI
Tested Reactivity Hu, Ms, Rat, Bov, Dog, Gpig, Hm, Mk, Pig, Sheep, Xenopus laevis
Tested Application ICC/IF, IHC, IP, WB
Specificity Detects ~58kDa.
Host Rabbit
Clonality Polyclonal
Target Name PDI
Antigen Species Rat
Immunogen KLH-conjugated synthetic peptide from Rat PDI
Conjugation Un-conjugated
Alternate Names PDA2; Pancreas-specific protein disulfide isomerase; PDIR; PDIP; PDI; Protein disulfide-isomerase A2; PDIp; EC 5.3.4.1

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100
IHCAssay-dependent
IPAssay-dependent
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol
Preservative 0.09% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 64714 Human PDIA2

GeneID: 69191 Mouse PDIA2

Swiss-port # D3Z6P0 Mouse Protein disulfide-isomerase A2

Swiss-port # Q13087 Human Protein disulfide-isomerase A2

Gene Symbol Pdia2
Gene Full Name protein disulfide isomerase family A, member 2
Background Protein disulfide isomerases (EC 5.3.4.1), such as PDIP, are endoplasmic reticulum (ER) resident proteins that catalyze protein folding and thiol-disulfide interchange reactions (Desilva et al., 1996 [PubMed 8561901]).[supplied by OMIM, Mar 2008]
Function Acts as an intracellular estrogen-binding protein. May be involved in modulating cellular levels and biological functions of estrogens in the pancreas. May act as a chaperone that inhibits aggregation of misfolded proteins. [UniProt]
Cellular Localization Endoplasmic Reticulum, Endoplasmic reticulum lumen
Calculated MW 58 kDa
PTM The disulfide-linked homodimer exhibits an enhanced chaperone activity.
Glycosylated.

Images (3) Click the Picture to Zoom In

  • ARG22292 anti-PDI antibody ICC/IF image

    Immunocytochemistry: 2% Formaldehyde (20 min at RT) fixed HeLa cells stained with ARG22292 anti-PDI antibody (yellow) at 1:100 dilution (12 hours at 4°C). Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Magnification: 100x. Left: DAPI (blue) nuclear stain, Middle: Primary antibody, Right: Composite.

  • ARG22292 anti-PDI antibody WB image

    Western blot: 50 ng of PDI purified protein and 15 µg of Rat tissue/cell lysates stained with ARG22292 anti-PDI antibody at 1:4000 dilution.

  • ARG22292 anti-PDI antibody ICC/IF image

    Immunocytochemistry: 2% Formaldehyde (20 min at RT) fixed HeLa cells stained with ARG22292 anti-PDI antibody (green) at 1:100 dilution (12 hours at 4°C). Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Magnification: 20x. Left: DAPI (blue) nuclear stain, Middle: Primary antibody, Right: Composite.