ARG22213

anti-PDIA3 / ERp57 antibody [MaP.Erp57]

anti-PDIA3 / ERp57 antibody [MaP.Erp57] for Western blot,Immunohistochemistry,ICC/IF,Immunoprecipitation and Dog,Human,Monkey,Mouse,Rat,Bovine,Guinea pig,Hamster,Pig,Rabbit

Overview

Product Description Mouse Monoclonal antibody [Map.ERP57] recognizes PDIA3 / ERp57
Tested Reactivity Hu, Ms, Rat, Bov, Dog, Gpig, Hm, Mk, Pig, Rb
Tested Application ICC/IF, IHC, IP, WB
Specificity Detects ~57kDa.
Host Mouse
Clonality Monoclonal
Clone Map.ERP57
Isotype IgG1
Target Name PDIA3 / ERp57
Antigen Species Human
Immunogen Human recombinant ERp57
Conjugation Un-conjugated
Alternate Names EC 5.3.4.1; Disulfide isomerase ER-60; HEL-S-93n; GRP57; p58; Endoplasmic reticulum resident protein 60; ER protein 57; ER protein 60; ERp57; GRP58; P58; 58 kDa glucose-regulated protein; 58 kDa microsomal protein; ER60; HEL-S-269; Protein disulfide-isomerase A3; PI-PLC; ERp60; ERp61; HsT17083; Endoplasmic reticulum resident protein 57

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:100
IHC1:100
IPAssay-dependent
WB1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol
Preservative 0.09% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 14827 Mouse PDIA3

GeneID: 2923 Human PDIA3

GeneID: 29468 Rat PDIA3

Gene Symbol PDIA3
Gene Full Name protein disulfide isomerase family A, member 3
Background This gene encodes a protein of the endoplasmic reticulum that interacts with lectin chaperones calreticulin and calnexin to modulate folding of newly synthesized glycoproteins. The protein was once thought to be a phospholipase; however, it has been demonstrated that the protein actually has protein disulfide isomerase activity. It is thought that complexes of lectins and this protein mediate protein folding by promoting formation of disulfide bonds in their glycoprotein substrates. [provided by RefSeq, Jul 2008]
Cellular Localization Endoplasmic Reticulum, Endoplasmic reticulum lumen, Melanosome
Highlight Related products:
ERp57 antibodies; Anti-Mouse IgG secondary antibodies;
Related poster download:
Organelle Markers & Loading Control
Calculated MW 57 kDa

Images (2) Click the Picture to Zoom In

  • ARG22213 anti-PDIA3 / ERp57 antibody [MaP.Erp57] ICC/IF image

    Immunocytochemistry: 2% Formaldehyde (20 min at RT) fixed Heat Shocked HeLa cells stained with ARG22213 anti-PDIA3 / ERp57 antibody [MaP.Erp57] (green) at 1:100 dilution (12 hours at 4°C). Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Magnification: 100x. Left: DAPI (blue) nuclear stain, Middle: Primary antibody, Right: Composite.

  • ARG22213 anti-PDIA3 / ERp57 antibody [MaP.Erp57] WB image

    Western blot: Human cell lysates stained with ARG22213 anti-PDIA3 / ERp57 antibody [MaP.Erp57] at 1:1000 dilution.