ARG53458

anti-PHLPP2 antibody [SP220]

anti-PHLPP2 antibody [SP220] for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Signaling Transduction antibody

Overview

Product Description Rabbit Monoclonal antibody [SP220] recognizes PHLPP2
Tested Reactivity Hu
Tested Application IHC-P, WB
Host Rabbit
Clonality Monoclonal
Clone SP220
Isotype IgG
Target Name PHLPP2
Antigen Species Human
Immunogen Synthetic peptide derived from human PHLPP2 protein.
Conjugation Un-conjugated
Alternate Names PH domain leucine-rich repeat-containing protein phosphatase 2; PHLPP-like; PH domain leucine-rich repeat-containing protein phosphatase-like; PHLPPL; EC 3.1.3.16

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:100
WB1:400
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Prostate, Prostate Adenocarcinoma, HEK293T Cell Lysate

Properties

Form Liquid
Purification Purified by protein A/G
Buffer PBS (pH 7.6), 1% BSA and < 0.1% Sodium azide
Preservative < 0.1% Sodium azide
Stabilizer 1% BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 23035 Human PHLPP2

Swiss-port # Q6ZVD8 Human PH domain leucine-rich repeat-containing protein phosphatase 2

Gene Symbol PHLPP2
Gene Full Name PH domain and leucine rich repeat protein phosphatase 2
Function Protein phosphatase that mediates dephosphorylation of 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. AKT1 regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of AKT1 triggers apoptosis and decreases cell proliferation. Also controls the phosphorylation of AKT3. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Inhibits cancer cell proliferation and may act as a tumor suppressor. [UniProt]
Cellular Localization Cytoplasm, Membrane, Nucleus
Research Area Signaling Transduction antibody
Calculated MW 147 kDa

Images (3) Click the Picture to Zoom In

  • ARG53458 anti-PHLPP2 antibody [SP220] WB image

    Western Blot: HEK 293T Cell Lysate stained with PHLPP2 antibody [SP220] (ARG53458)

  • ARG53458 anti-PHLPP2 antibody [SP220] IHC-P image

    Immunohistochemistry: Human Normal Prostate stained with PHLPP2 antibody [SP220] (ARG53458)

  • ARG53458 anti-PHLPP2 antibody [SP220] IHC-P image

    Immunohistochemistry: Human Prostate Adenocarcinoma stained with PHLPP2 antibody [SP220] (ARG53458)