anti-PI3 Kinase p85 alpha antibody [M253]

anti-PI3 Kinase p85 alpha antibody [M253] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human

Cancer antibody; Immune System antibody; Signaling Transduction antibody


Product Description Mouse Monoclonal antibody [M253] recognizes PI3 Kinase p85 alpha
Tested Reactivity Hu
Predict Reactivity Ms, Rat, Chk
Tested Application ICC/IF, IHC-P, WB
Specificity This antibody detects an 85 kDa band corresponding to the p85 subunit of PI3 Kinase in western blots of human A431 and Jurkat cells.
Host Mouse
Clonality Monoclonal
Clone M253
Isotype IgG2a
Target Name PI3 Kinase p85 alpha
Antigen Species Human
Immunogen Recombinant protein around the C-terminus of Human PI3 Kinase p85. This region is homologous to similar regions in Rat and Mouse PI3 Kinase p85.
Conjugation Un-conjugated
Alternate Names GRB1; PI3-kinase subunit p85-alpha; Phosphatidylinositol 3-kinase regulatory subunit alpha; IMD36; PtdIns-3-kinase regulatory subunit alpha; p85-ALPHA; p85; AGM7; PtdIns-3-kinase regulatory subunit p85-alpha; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha

Application Instructions

Application Suggestion
Tested Application Dilution
Application Note WB: Antibody is suggested to be diluted in 5% skimmed milk/Tris buffer with 0.04% Tween20 and incubated for 1 hour at room temperature.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Purification with Protein A.
Buffer 100 μl PBS, 50% Glycerol, 1 mg/ml BSA and 0.05% Sodium azide
Preservative 0.05% Sodium azide
Stabilizer 50% Glycerol, 1 mg/ml BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 5295 Human PIK3R1

Swiss-port # P27986 Human Phosphatidylinositol 3-kinase regulatory subunit alpha

Gene Symbol PIK3R1
Gene Full Name phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
Background Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]
Function Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling. Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress-and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement. [UniProt]
Highlight Related products:
PI3K p85 antibodies; Anti-Mouse IgG secondary antibodies;
Research Area Cancer antibody; Immune System antibody; Signaling Transduction antibody
Calculated MW 84 kDa
PTM Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.
Phosphorylated. Tyrosine phosphorylated in response to signaling by FGFR1, FGFR2, FGFR3 and FGFR4. Phosphorylated by CSF1R. Phosphorylated by ERBB4. Phosphorylated on tyrosine residues by TEK/TIE2. Dephosphorylated by PTPRJ. Phosphorylated by PIK3CA at Ser-608; phosphorylation is stimulated by insulin and PDGF. The relevance of phosphorylation by PIK3CA is however unclear (By similarity). Phosphorylated in response to KIT and KITLG/SCF. Phosphorylated by FGR.

Images (2) Click the Picture to Zoom In

  • ARG20571 anti-PI3 Kinase p85 alpha antibody [M253] WB image

    Western blot: A431 cells stained with ARG20571 anti-PI3 Kinase p85 alpha antibody [M253] at 1:1000 (Lane 1), 1:2000 (Lane 2), and 1:4000 (Lanes 3) dilutions.

  • ARG20571 anti-PI3 Kinase p85 alpha antibody [M253] ICC/IF image

    Immunocytochemistry: Aldehyde-fixed and NP-40-permeabilized A431 cells stained with ARG20571 anti-PI3 Kinase p85 alpha antibody [M253].