anti-PI3 Kinase p85 alpha antibody [M253]
anti-PI3 Kinase p85 alpha antibody [M253] for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Cancer antibody; Immune System antibody; Signaling Transduction antibody
|Product Description||Mouse Monoclonal antibody [M253] recognizes PI3 Kinase p85 alpha|
|Predict Reactivity||Ms, Rat, Chk|
|Tested Application||ICC/IF, IHC-P, WB|
|Specificity||This antibody detects an 85 kDa band corresponding to the p85 subunit of PI3 Kinase in western blots of human A431 and Jurkat cells.|
|Target Name||PI3 Kinase p85 alpha|
|Immunogen||Recombinant protein around the C-terminus of Human PI3 Kinase p85. This region is homologous to similar regions in Rat and Mouse PI3 Kinase p85.|
|Alternate Names||GRB1; PI3-kinase subunit p85-alpha; Phosphatidylinositol 3-kinase regulatory subunit alpha; IMD36; PtdIns-3-kinase regulatory subunit alpha; p85-ALPHA; p85; AGM7; PtdIns-3-kinase regulatory subunit p85-alpha; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha|
|Application Note||WB: Antibody is suggested to be diluted in 5% skimmed milk/Tris buffer with 0.04% Tween20 and incubated for 1 hour at room temperature.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Purification||Purification with Protein A.|
|Buffer||100 μl PBS, 50% Glycerol, 1 mg/ml BSA and 0.05% Sodium azide|
|Preservative||0.05% Sodium azide|
|Stabilizer||50% Glycerol, 1 mg/ml BSA|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||phosphoinositide-3-kinase, regulatory subunit 1 (alpha)|
|Background||Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]|
|Function||Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling. Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress-and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement. [UniProt]|
PI3K p85 antibodies; Anti-Mouse IgG secondary antibodies;
|Research Area||Cancer antibody; Immune System antibody; Signaling Transduction antibody|
|Calculated MW||84 kDa|
|PTM||Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.
Phosphorylated. Tyrosine phosphorylated in response to signaling by FGFR1, FGFR2, FGFR3 and FGFR4. Phosphorylated by CSF1R. Phosphorylated by ERBB4. Phosphorylated on tyrosine residues by TEK/TIE2. Dephosphorylated by PTPRJ. Phosphorylated by PIK3CA at Ser-608; phosphorylation is stimulated by insulin and PDGF. The relevance of phosphorylation by PIK3CA is however unclear (By similarity). Phosphorylated in response to KIT and KITLG/SCF. Phosphorylated by FGR.
Images (2) Click the Picture to Zoom In
ARG20571 anti-PI3 Kinase p85 alpha antibody [M253] WB image
Western blot: A431 cells stained with ARG20571 anti-PI3 Kinase p85 alpha antibody [M253] at 1:1000 (Lane 1), 1:2000 (Lane 2), and 1:4000 (Lanes 3) dilutions.
ARG20571 anti-PI3 Kinase p85 alpha antibody [M253] ICC/IF image
Immunocytochemistry: Aldehyde-fixed and NP-40-permeabilized A431 cells stained with ARG20571 anti-PI3 Kinase p85 alpha antibody [M253].