anti-PI3 Kinase p85 / p55 phospho (Tyr467 / 199) antibody

anti-PI3 Kinase p85 / p55 phospho (Tyr467 / 199) antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Monkey,Mouse,Rat

publication_link Publication1


Product Description Rabbit Polyclonal antibody recognizes PI3 Kinase p85 / p55 phospho (Tyr467 / 199)
Tested Reactivity Hu, Ms, Rat, Mk
Tested Application IHC-P, WB
Specificity This antibody detects endogenous levels of PI3 Kinase p85/p55 protein only when phosphorylated at Tyr467/199.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PI3 Kinase p85 / p55
Antigen Species Human
Immunogen Phosphospecific peptide around Tyr467/199 of Human PI3 Kinase p85/p55.
Conjugation Un-conjugated
Alternate Names GRB1; PI3-kinase subunit p85-alpha; Phosphatidylinositol 3-kinase regulatory subunit alpha; IMD36; PtdIns-3-kinase regulatory subunit alpha; p85-ALPHA; p85; AGM7; PtdIns-3-kinase regulatory subunit p85-alpha; PI3-kinase regulatory subunit alpha; PI3K regulatory subunit alpha; Phosphatidylinositol 3-kinase 85 kDa regulatory subunit alpha; PI3K p85; PI3K p55

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:100 - 1:300
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 0.02% Sodium azide, 50% Glycerol and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol and 0.5% BSA
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 18708 Mouse PIK3R1

GeneID: 25513 Rat PIK3R1

GeneID: 5295 Human PIK3R1

Gene Symbol PIK3R1
Gene Full Name phosphoinositide-3-kinase, regulatory subunit 1 (alpha)
Background Phosphatidylinositol 3-kinase phosphorylates the inositol ring of phosphatidylinositol at the 3-prime position. The enzyme comprises a 110 kD catalytic subunit and a regulatory subunit of either 85, 55, or 50 kD. This gene encodes the 85 kD regulatory subunit. Phosphatidylinositol 3-kinase plays an important role in the metabolic actions of insulin, and a mutation in this gene has been associated with insulin resistance. Alternative splicing of this gene results in four transcript variants encoding different isoforms. [provided by RefSeq, Jun 2011]
Function Binds to activated (phosphorylated) protein-Tyr kinases, through its SH2 domain, and acts as an adapter, mediating the association of the p110 catalytic unit to the plasma membrane. Necessary for the insulin-stimulated increase in glucose uptake and glycogen synthesis in insulin-sensitive tissues. Plays an important role in signaling in response to FGFR1, FGFR2, FGFR3, FGFR4, KITLG/SCF, KIT, PDGFRA and PDGFRB. Likewise, plays a role in ITGB2 signaling. Modulates the cellular response to ER stress by promoting nuclear translocation of XBP1 isoform 2 in a ER stress-and/or insulin-dependent manner during metabolic overloading in the liver and hence plays a role in glucose tolerance improvement. [UniProt]
Calculated MW 84 kDa
PTM Polyubiquitinated in T-cells by CBLB; which does not promote proteasomal degradation but impairs association with CD28 and CD3Z upon T-cell activation.
Phosphorylated. Tyrosine phosphorylated in response to signaling by FGFR1, FGFR2, FGFR3 and FGFR4. Phosphorylated by CSF1R. Phosphorylated by ERBB4. Phosphorylated on tyrosine residues by TEK/TIE2. Dephosphorylated by PTPRJ. Phosphorylated by PIK3CA at Ser-608; phosphorylation is stimulated by insulin and PDGF. The relevance of phosphorylation by PIK3CA is however unclear (By similarity). Phosphorylated in response to KIT and KITLG/SCF. Phosphorylated by FGR.

Images (1) Click the Picture to Zoom In

  • ARG66216 anti-PI3 Kinase p85 / p55 phospho (Tyr467 / 199) antibody WB image

    Western blot: COS7 cells treated with of without H2O2 stained with ARG66216 anti-PI3 Kinase p85 / p55 phospho (Tyr467 / 199) antibody at 1:1000 dilution.

Specific References

Acidic Microenvironment Regulates the Severity of Hepatic Ischemia/Reperfusion Injury by Modulating the Generation and Function of Tregs via the PI3K-mTOR Pathway

WB / Human

Gan Xiaojie et al.
Preprint.,  (2019)