ARG51669

anti-PKC theta phospho (Ser695) antibody

anti-PKC theta phospho (Ser695) antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat

Cell Biology and Cellular Response antibody; Gene Regulation antibody; Immune System antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes PKC theta phospho (Ser695)
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PKC theta
Antigen Species Human
Immunogen Peptide sequence around phosphorylation site of serine 695 (N-F-S(p)-F-M) derived from Human PKCθ.
Conjugation Un-conjugated
Alternate Names EC 2.7.11.13; PRKCT; Protein kinase C theta type; nPKC-theta

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:100
WB1:500 - 1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Buffer PBS (without Mg2+ and Ca2+, pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 18761 Mouse PRKCQ

GeneID: 5588 Human PRKCQ

Swiss-port # Q02111 Mouse Protein kinase C theta type

Swiss-port # Q04759 Human Protein kinase C theta type

Gene Symbol PRKCQ
Gene Full Name protein kinase C, theta
Background This is a calcium-independent, phospholipid-dependent, serine- and threonine-specific enzyme. Essential for T-cell receptor (TCR)-mediated T-cell activation, but is dispensable during TCR-dependent thymocyte development. Links the TCR signaling complex to the activation of NF-kappa-B in mature T lymphocytes. Required for interleukin-2 (IL2) production. PKC is activated by diacylglycerol which in turn phosphorylates a range of cellular proteins. PKC also serves as the receptor for phorbol esters, a class of tumor promoters.
Function Calcium-independent, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase that mediates non-redundant functions in T-cell receptor (TCR) signaling, including T-cells activation, proliferation, differentiation and survival, by mediating activation of multiple transcription factors such as NF-kappa-B, JUN, NFATC1 and NFATC2. In TCR-CD3/CD28-co-stimulated T-cells, is required for the activation of NF-kappa-B and JUN, which in turn are essential for IL2 production, and participates to the calcium-dependent NFATC1 and NFATC2 transactivation. Mediates the activation of the canonical NF-kappa-B pathway (NFKB1) by direct phosphorylation of CARD11 on several serine residues, inducing CARD11 association with lipid rafts and recruitment of the BCL10-MALT1 complex, which then activates IKK complex, resulting in nuclear translocation and activation of NFKB1. May also play an indirect role in activation of the non-canonical NF-kappa-B (NFKB2) pathway. In the signaling pathway leading to JUN activation, acts by phosphorylating the mediator STK39/SPAK and may not act through MAP kinases signaling. Plays a critical role in TCR/CD28-induced NFATC1 and NFATC2 transactivation by participating in the regulation of reduced inositol 1,4,5-trisphosphate generation and intracellular calcium mobilization. After costimulation of T-cells through CD28 can phosphorylate CBLB and is required for the ubiquitination and subsequent degradation of CBLB, which is a prerequisite for the activation of TCR. During T-cells differentiation, plays an important role in the development of T-helper 2 (Th2) cells following immune and inflammatory responses, and, in the development of inflammatory autoimmune diseases, is necessary for the activation of IL17-producing Th17 cells. May play a minor role in Th1 response. Upon TCR stimulation, mediates T-cell protective survival signal by phosphorylating BAD, thus protecting T-cells from BAD-induced apoptosis, and by up-regulating BCL-X(L)/BCL2L1 levels through NF-kappa-B and JUN pathways. In platelets, regulates signal transduction downstream of the ITGA2B, CD36/GP4, F2R/PAR1 and F2RL3/PAR4 receptors, playing a positive role in 'outside-in' signaling and granule secretion signal transduction. May relay signals from the activated ITGA2B receptor by regulating the uncoupling of WASP and WIPF1, thereby permitting the regulation of actin filament nucleation and branching activity of the Arp2/3 complex. May mediate inhibitory effects of free fatty acids on insulin signaling by phosphorylating IRS1, which in turn blocks IRS1 tyrosine phosphorylation and downstream activation of the PI3K/AKT pathway. Phosphorylates MSN (moesin) in the presence of phosphatidylglycerol or phosphatidylinositol. Phosphorylates PDPK1 at 'Ser-504' and 'Ser-532' and negatively regulates its ability to phosphorylate PKB/AKT1. [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30042 Phospho IRS1 (pS307) / Phospho PKC theta (pS695) Antibody Duo
Related products:
PKC theta antibodies; PKC theta Duos / Panels; Anti-Rabbit IgG secondary antibodies;
Research Area Cell Biology and Cellular Response antibody; Gene Regulation antibody; Immune System antibody; Signaling Transduction antibody
Calculated MW 82 kDa
PTM Autophosphorylation at Thr-219 is required for targeting to the TCR and cellular function of PRKCQ upon antigen receptor ligation. Following TCR stimulation, phosphorylated at Tyr-90 and Ser-685.

Images (2) Click the Picture to Zoom In

  • ARG51669 anti-PKC theta phospho (Ser695) antibody WB image

    Western blot: Extracts from 293 cells untreated or treated with PMA stained with ARG51669 anti-PKC theta phospho (Ser695) antibody.

  • ARG51669 anti-PKC theta phospho (Ser695) antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human lung carcinoma tissue stained with ARG51669 anti-PKC theta phospho (Ser695) antibody (left) or the same antibody preincubated with blocking peptide (right).