anti-PLN / Phospholamban phospho (Thr17) antibody

anti-PLN / Phospholamban phospho (Thr17) antibody for Dot blot,Western blot and Human,Rat

publication_link Publication1


Product Description Rabbit Polyclonal antibody recognizes PLN / Phospholamban phospho (Thr17)
Tested Reactivity Hu, Rat
Tested Application Dot, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PLN / Phospholamban
Antigen Species Human
Immunogen KLH-conjugated phosphospecific peptide around Thr17 of Human PLN.
Conjugation Un-conjugated
Alternate Names CMH18; PLB; CMD1P; Cardiac phospholamban

Application Instructions

Application Suggestion
Tested Application Dilution
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Spontaneous hypertensive rat heart
Observed Size 11 and 26 kDa


Form Liquid
Purification Purification with Protein A.
Buffer PBS and 0.09% (W/V) Sodium azide.
Preservative 0.09% (W/V) Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 5350 Human PLN

GeneID: 64672 Rat PLN

Swiss-port # P26678 Human Cardiac phospholamban

Swiss-port # P61016 Rat Cardiac phospholamban

Gene Symbol PLN
Gene Full Name phospholamban
Background The protein encoded by this gene is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure. [provided by RefSeq, Jul 2008]
Function Reversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca(2+). Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation. [UniProt]
Cellular Localization Endoplasmic reticulum membrane; Single-pass membrane protein. Sarcoplasmic reticulum membrane; Single-pass membrane protein. Mitochondrion membrane; Single-pass membrane protein. Membrane; Single-pass membrane protein. Note=Colocalizes with HAX1 at the endoplasmic reticulum (PubMed:17241641). Colocalizes with DMPK a the sarcoplasmic reticulum (PubMed:15598648). [UniProt]
Calculated MW 6 kDa
PTM Phosphorylation by PKA abolishes the inhibition of ATP2A2-mediated calcium uptake. Phosphorylated at Thr-17 by CaMK2, and in response to beta-adrenergic stimulation. Phosphorylation by DMPK may stimulate sarcoplasmic reticulum calcium uptake in cardiomyocytes. [UniProt]

Images (2) Click the Picture to Zoom In

  • ARG41951 anti-PLN / Phospholamban phospho (Thr17) antibody WB image

    Western blot: Spontaneous hypertensive (SH) rat heart and normal rat heart tissue lysates stained with ARG41951 anti-PLN / Phospholamban phospho (Thr17) antibody.

  • ARG41951 anti-PLN / Phospholamban phospho (Thr17) antibody Dot image

    Dot blot: Analysis of ARG41951 anti-PLN / Phospholamban phospho (Thr17) antibody on nitrocellulose membrane. 50 ng of Phosphopeptide or Non-phosphopeptide per dot were adsorbed. Antibody working concentrations are 0.5 µg/ml.

Specific References

Consuming oxidative frying oil impairs cardiac energy production and calcium recycling, causing cardiac hypertrophy, fibrosis and diastolic dysfunction in male Sprague Dawley rats

WB / Rat

Yu-Shun Lin et al.
J Nutr Biochem.,  (2021)