ARG20170

anti-PPAR alpha antibody

anti-PPAR alpha antibody for Immunoprecipitation,Western blot and Bovine,Human,Mouse,Pig,Rat

Overview

Product Description

Rabbit Polyclonal antibody recognizes PPAR alpha

Tested Reactivity Hu, Ms, Rat, Bov, Pig
Tested Application IP, WB
Specificity The antibody recognizes ~52 kDa PPARα in human sample. A ~28 kDa cleavage fragment can also be detected in samples of human, mouse, and rat origins.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PPAR alpha
Species Human
Immunogen Synthetic peptide surrounding amino acid 25 of human PPARα
Conjugation Un-conjugated
Alternate Names hPPAR; NR1C1; Peroxisome proliferator-activated receptor alpha; PPAR; Nuclear receptor subfamily 1 group C member 1; PPAR-alpha; PPARalpha

Application Instructions

Application Suggestion
Tested Application Dilution
IP25 µg/ml
WB0.5-6 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 52 kDa

Properties

Form Liquid
Purification Affinity Purified Antibody
Buffer PBS, 30% Glycerol, 0.5% BSA and 0.01% Thimerosal
Preservative 0.01% Thimerosal
Stabilizer 30% Glycerol, 0.5% BSA
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 19013 Mouse PPARA

GeneID: 25747 Rat PPARA

GeneID: 5465 Human PPARA

Background PPAR (Peroxisome proliferator-activated receptor) is a member of the nuclear hormone receptor superfamily and functions as a transcriptional activator. PPARα is preferentially expressed in liver, skeletal muscle, heart and kidney. PPARα is involved in regulation of lipid homeostasis. Its ligands include fatty acids, NSAIDs, prostaglandins, leukotriene B4, etc. PPARα transcriptionally regulates a variety of genes for enzymes and proteins involved in fatty acid metabolism and oxidation.

Images (1) Click the Picture to Zoom In

  • ARG20170 anti-PPARa antibody WB image

    Western Blot: 1,2. Jurkat cell lysate 3. Rat liver tissue lysate 4. Rat kidney tissue lysate stained with anti-PPAR alpha antibody (ARG20170).

    The antibody recognizes ~52 kDa PPARα in human sample. A ~28 kDa cleavage fragment can also be detected in samples of human, mouse, and rat origins.