ARG45462
anti-PPIE antibody [7F2]
anti-PPIE antibody [7F2] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Mouse Monoclonal antibody [7F2] recognizes PPIE |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 7F2 |
| Isotype | IgG2a |
| Target Name | PPIE |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human PPIE. |
| Conjugation | Un-conjugated |
| Alternate Names | Cyclophilin-33; CYP-33; Cyclophilin E; PPIase E; EC 5.2.1.8; Peptidyl-prolyl cis-trans isomerase E; CYP33; Rotamase E |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
| Observed Size | 35 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9QZH3 Mouse Peptidyl-prolyl cis-trans isomerase E Swiss-port # Q9UNP9 Human Peptidyl-prolyl cis-trans isomerase E |
|---|---|
| Gene Symbol | PPIE |
| Gene Full Name | peptidylprolyl isomerase E (cyclophilin E) |
| Background | The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein contains a highly conserved cyclophilin (CYP) domain as well as an RNA-binding domain. It was shown to possess PPIase and protein folding activities, and it also exhibits RNA-binding activity. Alternative splicing results in multiple transcript variants. A related pseudogene, which is also located on chromosome 1, has been identified. [provided by RefSeq, Aug 2010] |
| Function | PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. Combines RNA-binding and PPIase activities. May be involved in muscle- and brain-specific processes. May be involved in pre-mRNA splicing. [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 33 kDa |
| PTM | Phosphoprotein. [UniProt] |
Images (7) Click the Picture to Zoom In
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ARG45462 anti-PPIE antibody [7F2] IHC-P image
Immunohistochemistry: Human placenta stained with ARG45462 anti-PPIE antibody [7F2] at 2 μg/ml dilution.
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ARG45462 anti-PPIE antibody [7F2] ICC/IF image
Immunofluorescence: Hela stained with ARG45462 anti-PPIE antibody [7F2] at 5 μg/ml dilution.
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ARG45462 anti-PPIE antibody [7F2] WB image
Western blot: HEK293 stained with ARG45462 anti-PPIE antibody [7F2] at 0.5 μg/ml dilution.
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ARG45462 anti-PPIE antibody [7F2] FACS image
Flow Cytometry: JK stained with ARG45462 anti-PPIE antibody [7F2] at 1 µg/10^6 cells dilution.
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ARG45462 anti-PPIE antibody [7F2] IHC-P image
Immunohistochemistry: Rat cardiac stained with ARG45462 anti-PPIE antibody [7F2] at 2 μg/ml dilution.
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ARG45462 anti-PPIE antibody [7F2] WB image
Western blot: Rat lung stained with ARG45462 anti-PPIE antibody [7F2] at 0.5 μg/ml dilution.
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ARG45462 anti-PPIE antibody [7F2] WB image
Western blot: HEPA1-6 stained with ARG45462 anti-PPIE antibody [7F2] at 0.5 μg/ml dilution.
