ARG46398
anti-PPM1D antibody
anti-PPM1D antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes PPM1D |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PPM1D |
| Antigen Species | Human |
| Immunogen | A 18 amino acid synthetic peptide within the last 50 amino acids of human PPM1D. |
| Conjugation | Un-conjugated |
| Alternate Names | PPM1D; WIP1; PP2C-DELTA; Protein phosphatase magnesium-dependent 1 delta; EC 3.1.3.16; Protein phosphatase 1D; p53-induced protein phosphatase 1; PP2C-delta; Protein phosphatase 2C isoform delta |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Observed Size | 68 kDa |
Properties
| Purification | Affinity chromatography purified |
|---|---|
| Buffer | PBS and 0.02% Sodium azide. |
| Preservative | 0.02% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PPM1D |
| Gene Full Name | protein phosphatase, Mg2+/Mn2+ dependent, 1D |
| Background | The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The expression of this gene is induced in a p53-dependent manner in response to various environmental stresses. While being induced by tumor suppressor protein TP53/p53, this phosphatase negatively regulates the activity of p38 MAP kinase, MAPK/p38, through which it reduces the phosphorylation of p53, and in turn suppresses p53-mediated transcription and apoptosis. This phosphatase thus mediates a feedback regulation of p38-p53 signaling that contributes to growth inhibition and the suppression of stress induced apoptosis. This gene is located in a chromosomal region known to be amplified in breast cancer. The amplification of this gene has been detected in both breast cancer cell line and primary breast tumors, which suggests a role of this gene in cancer development. [provided by RefSeq, Jul 2008] |
| Function | Required for the relief of p53-dependent checkpoint mediated cell cycle arrest. Binds to and dephosphorylates 'Ser-15' of TP53 and 'Ser-345' of CHEK1 which contributes to the functional inactivation of these proteins. [UniProt] |
| Calculated MW | 67 kDa |
Images (2) Click the Picture to Zoom In
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ARG46398 anti-PPM1D antibody IHC-P image
Immunohistochemistry: Human lung carcinoma stained with ARG46398 anti-PPM1D antibody.
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ARG46398 anti-PPM1D antibody WB image
Western blot: 3T3 stained with ARG46398 anti-PPM1D antibody.
