ARG54048

anti-PPP1A antibody

anti-PPP1A antibody for Western blot,ICC/IF and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody

Overview

Product Description Mouse Monoclonal antibody recognizes PPP1CA
Tested Reactivity Hu
Tested Application ICC/IF, WB
Host Mouse
Clonality Monoclonal
Isotype IgG1
Target Name PPP1A
Antigen Species Human
Immunogen Purified recombinant human PPP1A protein fragments expressed in E.coli.
Conjugation Un-conjugated
Alternate Names PP-1A; PPP1A; Serine/threonine-protein phosphatase PP1-alpha catalytic subunit; EC 3.1.3.16; PP1A; PP1alpha

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50
WB1:1000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Observed Size 37 kDa

Properties

Form Liquid
Purification Affinity purified
Buffer 0.1M Tris-Glycine (pH 7.4), 150 mM NaCl, 0.2% Sodium azide and 50% Glycerol
Preservative 0.2% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 5499 Human PPP1CA

Swiss-port # P62136 Human Serine/threonine-protein phosphatase PP1-alpha catalytic subunit

Gene Symbol PPP1CA
Gene Full Name protein phosphatase 1, catalytic subunit, alpha isozyme
Background Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role. in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase.
Function Protein phosphatase that associates with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate hundreds of biological targets. Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. Involved in regulation of ionic conductances and long-term synaptic plasticity. May play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca(2+)/calmodulin dependent protein kinase II. Component of the PTW/PP1 phosphatase complex, which plays a role in the control of chromatin structure and cell cycle progression during the transition from mitosis into interphase. Regulates NEK2 function in terms of kinase activity and centrosome number and splitting, both in the presence and absence of radiation-induced DNA damage. Regulator of neural tube and optic fissure closure, and enteric neural crest cell (ENCCs) migration during development. In balance with CSNK1D and CSNK1E, determines the circadian period length, through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. May dephosphorylate CSNK1D and CSNK1E. Dephosphorylates the 'Ser-418' residue of FOXP3 in regulatory T-cells (Treg) from patients with rheumatoid arthritis, thereby inactivating FOXP3 and rendering Treg cells functionally defective (PubMed:23396208). [UniProt]
Cellular Localization Cytoplasm. Nucleus
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Gene Regulation antibody; Metabolism antibody; Signaling Transduction antibody
Calculated MW 38 kDa
PTM Phosphorylated. Dephosphorylated at Thr-320 in the presence of ionizing radiation.

Images (2) Click the Picture to Zoom In

  • ARG54048 anti-PPP1A antibody ICC/IF image

    Immunofluorescence: HeLa cells stained with ARG54048 anti-PPP1A antibody at 1:50 dilution.

  • ARG54048 anti-PPP1A antibody WB image

    Western blot: MCF7 cell lysate stained with ARG54048 anti-PPP1A antibody at 1:1000 dilution.