anti-PRAME antibody [SQab30314]

anti-PRAME antibody [SQab30314] for IHC-Formalin-fixed paraffin-embedded sections and Human


Product Description Recombinant rabbit Monoclonal antibody [SQab30314] recognizes PRAME
Tested Reactivity Hu
Tested Application IHC-P
Host Rabbit
Clonality Monoclonal
Clone SQab30314
Isotype IgG
Target Name PRAME
Antigen Species Human
Immunogen Synthetic peptide of Human PRAME.
Conjugation Un-conjugated
Protein Full Name Melanoma antigen preferentially expressed in tumors
Alternate Names PRAME, PRAME Nuclear Receptor Transcriptional Regulator, CT130, MAPE, Melanoma Antigen Preferentially Expressed In Tumors, Preferentially Expressed Antigen In Melanoma, Preferentially Expressed Antigen Of Melanoma, Cancer/Testis Antigen 130, Opa-Interacting Protein 4, OIP-4, OIP4, Opa-Interacting Protein OIP4

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:100
Application Note IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0). Incubate the samples at RT (18-25°C) for 35 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Human malignant melanoma


Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05%BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05%BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Gene Symbol PRAME
Gene Full Name PRAME Nuclear Receptor Transcriptional Regulator
Background This gene encodes an antigen that is preferentially expressed in human melanomas and that is recognized by cytolytic T lymphocytes. It is not expressed in normal tissues, except testis. The encoded protein acts as a repressor of retinoic acid receptor, and likely confers a growth advantage to cancer cells via this function. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014]
Function Substrate-recognition component of a Cul2-RING (CRL2) E3 ubiquitin-protein ligase complex, which mediates ubiquitination of target proteins, leading to their degradation.The CRL2(PRAME) complex mediates ubiquitination and degradation of truncated MSRB1/SEPX1 selenoproteins produced by failed UGA/Sec decoding. [Uniprot]
Cellular Localization Cell membrane, Chromosome, Cytoplasm, Golgi apparatu, Membran, Nucleus
Calculated MW 58 kDa
PTM Phosphoprotein.[Uniprot]

Images (1) Click the Picture to Zoom In

  • ARG67043 anti-PRAME antibody [SQab30314] IHC-P image

    Immunohistochemistry: Formalin-fixed and paraffin-embedded human malignant melanoma stained with ARG67043 anti-PRAME antibody [SQab30314].