ARG45222
anti-PRMT3 antibody
anti-PRMT3 antibody for Flow cytometry,ICC/IF,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes PRMT3 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | PRMT3 |
| Antigen Species | Human |
| Immunogen | Recombinant protein containing to human PRMT3. |
| Conjugation | Un-conjugated |
| Alternate Names | PRMT3; Protein Arginine Methyltransferase 3; HRMT1L3; Heterogeneous Nuclear Ribonucleoprotein Methyltransferase-Like Protein 3; Protein Arginine N-Methyltransferase 3; HMT1 HnRNP Methyltransferase-Like 3 (S. Cerevisiae); HMT1 HnRNP Methyltransferase-Like 3; EC 2.1.1.319; EC 2.1.1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Observed Size | 60-70 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl and 4% Trehalose. |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O60678 Human Protein arginine N-methyltransferase 3 |
|---|---|
| Gene Symbol | PRMT3 |
| Gene Full Name | Protein Arginine Methyltransferase 3 |
| Background | This gene belongs to the protein arginine methyltransferase (PRMT) family. The encoded enzyme catalyzes the methylation of guanidino nitrogens of arginyl residues of proteins. The enzyme acts on 40S ribosomal protein S2 (rpS2), which is its major in-vivo substrate, and is involved in the proper maturation of the 80S ribosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013] |
| Function | Protein-arginine N-methyltransferase that catalyzes both the monomethylation and asymmetric dimethylation of the guanidino nitrogens of arginine residues in target proteins, and therefore falls into the group of type I methyltransferases (Probable). May regulate retinoic acid synthesis and signaling by inhibiting ALDH1A1 retinal dehydrogenase activity. [UniProt] |
| Cellular Localization | Cytoplasm. [UniProt] |
| Calculated MW | 60 kDa |
| PTM | Acetylation; Phosphoprotein. [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG45222 anti-PRMT3 antibody ICC/IF image
Immunofluorescence: Caco-2 stained with ARG45222 anti-PRMT3 antibody at 5 ug/ml dilution.
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ARG45222 anti-PRMT3 antibody WB image
Western blot: MCF-7, HepG2, and Hela stained with ARG45222 anti-PRMT3 antibody at 0.5 μg/ml dilution.
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ARG45222 anti-PRMT3 antibody FACS image
Flow Cytometry: K562 stained with ARG45222 anti-PRMT3 antibody at 1 µg/10^6 cells dilution.
