ARG59786

anti-PRPF3 antibody

anti-PRPF3 antibody for Western blot,IHC-Formalin-fixed paraffin-embedded sections,ICC/IF and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes PRPF3
Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PRPF3
Species Human
Immunogen Recombinant fusion protein corresponding to aa. 1-280 of Human PRPF3 (NP_004689.1).
Conjugation Un-conjugated
Full Name pre-mRNA processing factor 3
Alternate Names Pre-mRNA-splicing factor 3; Prp3p; SNRNP90; U4/U6 small nuclear ribonucleoprotein Prp3; PRP3; hPrp3; U4/U6 snRNP 90 kDa protein; HPRP3; HPRP3P; RP18

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Mouse testis and BT-474
Calculated MW 78 kDa
Observed Size 100 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 70767 Mouse PRPF3

GeneID: 9129 Human PRPF3

Swiss-port # O43395 Human U4/U6 small nuclear ribonucleoprotein Prp3

Swiss-port # Q922U1 Mouse U4/U6 small nuclear ribonucleoprotein Prp3

Gene Symbol PRPF3
Gene Full Name pre-mRNA processing factor 3
Background The removal of introns from nuclear pre-mRNAs occurs on complexes called spliceosomes, which are made up of 4 small nuclear ribonucleoprotein (snRNP) particles and an undefined number of transiently associated splicing factors. This gene product is one of several proteins that associate with U4 and U6 snRNPs. Mutations in this gene are associated with retinitis pigmentosa-18. [provided by RefSeq, Jul 2008]
Function Participates in pre-mRNA splicing. Part of the U4/U5/U6 tri-snRNP complex, one of the building blocks of the spliceosome. [UniProt]
Cellular Localization Nucleus speckle. Note=Colocalizes with spliceosomal snRNPs. [UniProt]
Highlight Related products:
PRPF3 antibodies; Anti-Rabbit IgG secondary antibodies;
Related news:
Gene therapy for retinitis pigmentosa (RP)
PTM Ubiquitinated. Undergoes 'Lys-63'-linked polyubiquitination by PRPF19 and deubiquitination by USP4. 'Lys-63'-linked ubiquitination increases the affinity for PRPF8 and may regulate the assembly of the U4/U6-U5 tri-snRNP complex. [UniProt]

Images (4) Click the Picture to Zoom In

  • ARG59786 anti-PRPF3 antibody ICC/IF image

    Immunofluorescence: U2OS cells stained with ARG59786 anti-PRPF3 antibody.

  • ARG59786 anti-PRPF3 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human stomach stained with ARG59786 anti-PRPF3 antibody at 1:100 dilution.

  • ARG59786 anti-PRPF3 antibody WB image

    Western blot: 25 µg of Mouse testis and BT-474 cell lysates stained with ARG59786 anti-PRPF3 antibody at 1:1000 dilution.

  • ARG59786 anti-PRPF3 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Rat brain stained with ARG59786 anti-PRPF3 antibody at 1:100 dilution.