anti-PRR7 antibody [TRAP3/10]
anti-PRR7 antibody [TRAP3/10] for ICC/IF,Western blot and Human,Mouse,Rat
Mouse Monoclonal antibody [TRAP3/10] recognizes PRR7
|Tested Reactivity||Hu, Ms, Rat|
|Tested Application||ICC/IF, WB|
|Specificity||The clone TRAP3/10 recognizes an epitope located in the C-terminal part of the intracellular domain of PRR7/TRAP3 (amino acids 126-253 of human PRR7 / TRAP3), a 28 kDa proline-rich membrane protein presumably associated with NMDA receptor complex.|
|Immunogen||Recombinant C-terminal half of the intracellular domain of human PRR7/TRAP3 (amino acids 126-253)|
|Alternate Names||Synaptic proline-rich membrane protein; Proline-rich protein 7|
|Application Note||ICC/IF: Cell culture fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton-X100.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Positive Control||WB: Murine brain lysate (red. Laemmli buffer)|
|Purification||Purified from ascites by protein-A affinity chromatography.|
|Purity||> 95% (by SDS-PAGE)|
|Buffer||PBS (pH 7.4) and 15 mM Sodium azide|
|Preservative||15 mM Sodium azide|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||proline rich 7 (synaptic)|
|Background||PRR7/TRAP3 (proline-rich 7, transmembrane adaptor protein 3) is a 28 kDa transmembrane adaptor protein ubiquitously expressed at low level (most in brain). Its amino acid sequence is extremely conserved among mammalian and other species. PRR7/TRAP3 contains potential palmitoylation motif and is found in lipid rafts. It is a part of the complex postsynaptic density fraction in neurons and associates with PSD-95, NMDA receptor and probably other proteins. The intracellular domain of PRR7/TRAP3 contains several tyrosines, a proline-rich sequence, and a C-terminal PDZ-binding motif. So far nothing is known about function of this protein. It may be involved in regulation of some receptor signaling and in formation of neurologic and immunologic synapse.|
|Research Area||Neuroscience antibody|
|Calculated MW||31 kDa|
Images (2) Click the Picture to Zoom In
ARG53960 anti-PRR7 antibody [TRAP3/10] ICC/IF image
Immunofluorescence: HEK-293 cells cotransfected with PRR7 / TRAP3 and GFP-PSD-95 stained with ARG53960 anti-PRR7 antibody [TRAP3/10] (red)
Co-stained with GFP (green). Cell nuclei was stained with DAPI (blue).
ARG53960 anti-PRR7 antibody [TRAP3/10] WB image
Western blot: Murine brain lysate stained with ARG53960 anti-PRR7 antibody [TRAP3/10].