ARG53960

anti-PRR7 antibody [TRAP3/10]

anti-PRR7 antibody [TRAP3/10] for ICC/IF,Western blot and Human,Mouse,Rat

Neuroscience antibody

Overview

Product Description

Mouse Monoclonal antibody [TRAP3/10] recognizes PRR7

Tested Reactivity Hu, Ms, Rat
Tested Application ICC/IF, WB
Specificity The clone TRAP3/10 recognizes an epitope located in the C-terminal part of the intracellular domain of PRR7/TRAP3 (amino acids 126-253 of human PRR7 / TRAP3), a 28 kDa proline-rich membrane protein presumably associated with NMDA receptor complex.
Host Mouse
Clonality Monoclonal
Clone TRAP3/10
Isotype IgG2a
Target Name PRR7
Antigen Species Human
Immunogen Recombinant C-terminal half of the intracellular domain of human PRR7/TRAP3 (amino acids 126-253)
Conjugation Un-conjugated
Alternate Names Synaptic proline-rich membrane protein; Proline-rich protein 7

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF10 µg/ml
WB1 µg/ml
Application Note ICC/IF: Cell culture fixed with 4% paraformaldehyde, permeabilized with 0.1% Triton-X100.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control WB: Murine brain lysate (red. Laemmli buffer)

Properties

Form Liquid
Purification Purified from ascites by protein-A affinity chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 432763 Mouse PRR7

GeneID: 498704 Rat PRR7

GeneID: 80758 Human PRR7

Gene Symbol PRR7
Gene Full Name proline rich 7 (synaptic)
Background PRR7/TRAP3 (proline-rich 7, transmembrane adaptor protein 3) is a 28 kDa transmembrane adaptor protein ubiquitously expressed at low level (most in brain). Its amino acid sequence is extremely conserved among mammalian and other species. PRR7/TRAP3 contains potential palmitoylation motif and is found in lipid rafts. It is a part of the complex postsynaptic density fraction in neurons and associates with PSD-95, NMDA receptor and probably other proteins. The intracellular domain of PRR7/TRAP3 contains several tyrosines, a proline-rich sequence, and a C-terminal PDZ-binding motif. So far nothing is known about function of this protein. It may be involved in regulation of some receptor signaling and in formation of neurologic and immunologic synapse.
Research Area Neuroscience antibody
Calculated MW 31 kDa

Images (2) Click the Picture to Zoom In

  • ARG53960 anti-PRR7 antibody [TRAP3/10] ICC/IF image

    Immunofluorescence: HEK-293 cells cotransfected with PRR7 / TRAP3 and GFP-PSD-95 stained with ARG53960 anti-PRR7 antibody [TRAP3/10] (red)
    Co-stained with GFP (green). Cell nuclei was stained with DAPI (blue).

  • ARG53960 anti-PRR7 antibody [TRAP3/10] WB image

    Western blot: Murine brain lysate stained with ARG53960 anti-PRR7 antibody [TRAP3/10].