anti-PSA (free) antibody [103] (HRP)

anti-PSA (free) antibody [103] (HRP) for ELISA and Human

Cancer antibody; Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody
publication_link Publication1


Product Description HRP-conjugated Mouse Monoclonal antibody [103] recognizes PSA (free)
Tested Reactivity Hu
Tested Application ELISA
Specificity This monoclonal antibody reacts with human free PSA, but does not react with complexed PSA.
Host Mouse
Clonality Monoclonal
Clone 103
Isotype IgG2a, kappa
Target Name PSA (free)
Antigen Species Human
Immunogen Purified human PSA
Conjugation HRP
Alternate Names Prostate-specific antigen; Gamma-seminoprotein; APS; PSA; EC; Kallikrein-3; P-30 antigen; Semenogelase; hK3; Seminin; KLK2A1

Application Instructions

Application Note ELISA: In combination with a free PSA specific capture antibody (Cat. No.: ARG10190), this HRP conjugated antibody can be used as tracer antibody in a sandwich ELISA for human free PSA detection.

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Protein G affinity purified
Buffer 0.01M PBS (pH 7.2) and 50% Glycerol
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Keep the antibody in the dark and keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 354 Human KLK3

Swiss-port # P07288 Human Prostate-specific antigen

Gene Symbol KLK3
Gene Full Name kallikrein-related peptidase 3
Background Free prostate specific antigen (fPSA) is the unbounded form of PSA. Study has shown that low percentage of fPSA in total PSA (tPSA) is associated with high risk of prostate cancer. Using percentage of fPSA in tPSA can enhance the predication of prostate cancer and avoid unnecessary biopsies in prostate screen.
Function Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum. [UniProt]
Highlight Related Antibody Duos and Panels:
ARG30067 Free Prostate Specific Antigen ELISA Antibody Duo
Related products:
PSA antibodies; PSA ELISA Kits; PSA Duos / Panels; Anti-Mouse IgG secondary antibodies;
Research Area Cancer antibody; Controls and Markers antibody; Neuroscience antibody; Signaling Transduction antibody
Calculated MW 29 kDa

Specific References

A novel and highly sensitive sandwich-type immunosensor for prostate-specific antigen detection based on MWCNTs-Fe3O4 nanocomposite

ELISA / Human

AliShamsazar et al.
Sensors & Actuators: B. Chemical.,  (2021)