anti-PSAP antibody [SQab1899]
anti-PSAP antibody [SQab1899] for IHC-Formalin-fixed paraffin-embedded sections and Human
|Product Description||Recombinant Rabbit Monoclonal antibody [SQab1899] recognizes PSAP|
|Immunogen||Synthetic peptide within aa. 1-100 of Human PSAP.|
|Alternate Names||Glucosylceramidase activator; SAP-1; Sphingolipid activator protein 1; Component C; Protein C; Proactivator polypeptide; Protein A; Sphingolipid activator protein 2; Cerebroside sulfate activator; A1 activator; Prosaposin; Dispersin; SAP-2; Co-beta-glucosidase; CSAct; SAP1; GLBA; Sulfatide/GM1 activator|
|Application Note||IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0), primary antibody incubate at RT for 30 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
|Purification||Purification with Protein A.|
|Buffer||PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA.|
|Preservative||0.01% Sodium azide|
|Stabilizer||40% Glycerol and 0.05% BSA|
|Storage instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||prosaposin|
|Background||This gene encodes a highly conserved glycoprotein which is a precursor for 4 cleavage products: saposins A, B, C, and D. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities. Mutations in this gene have been associated with Gaucher disease, Tay-Sachs disease, and metachromatic leukodystrophy. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]|
|Function||Saposin-A and saposin-C stimulate the hydrolysis of glucosylceramide by beta-glucosylceramidase (EC 126.96.36.199) and galactosylceramide by beta-galactosylceramidase (EC 188.8.131.52). Saposin-C apparently acts by combining with the enzyme and acidic lipid to form an activated complex, rather than by solubilizing the substrate.
Saposin-B stimulates the hydrolysis of galacto-cerebroside sulfate by arylsulfatase A (EC 184.108.40.206), GM1 gangliosides by beta-galactosidase (EC 220.127.116.11) and globotriaosylceramide by alpha-galactosidase A (EC 18.104.22.168). Saposin-B forms a solubilizing complex with the substrates of the sphingolipid hydrolases.
Saposin-D is a specific sphingomyelin phosphodiesterase activator (EC 22.214.171.124).
Prosaposin: Behaves as a myelinotrophic and neurotrophic factor, these effects are mediated by its G-protein-coupled receptors, GPR37 and GPR37L1, undergoing ligand-mediated internalization followed by ERK phosphorylation signaling.
Saposins are specific low-molecular mass non-enzymic proteins, they participate in the lysosomal degradation of sphingolipids, which takes place by the sequential action of specific hydrolases. [UniProt]
PSAP antibodies; Anti-Rabbit IgG secondary antibodies;
Cancer Pathology Markers (SQ clones)
|PTM||The lysosomal precursor is proteolytically processed to 4 small peptides, which are similar to each other and are sphingolipid hydrolase activator proteins.
N-linked glycans show a high degree of microheterogeneity.
The one residue extended Saposin-B-Val is only found in 5% of the chains. [UniProt]
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