ARG67379
anti-PSAP antibody [SQab30414]
anti-PSAP antibody [SQab30414] for IHC-Formalin-fixed paraffin-embedded sections and Human
Overview
| Product Description | Rabbit Monoclonal antibody [SQab30414] recognizes PSAP |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IHC-P |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SQab30414 |
| Isotype | IgG |
| Target Name | PSAP |
| Immunogen | Synthetic peptide |
| Conjugation | Un-conjugated |
| Alternate Names | PSAP; Prosaposin; GLBA; SAP1; Sphingolipid Activator Protein-1; Sphingolipid Activator Protein-2; Proactivator Polypeptide; Saposin-A; Saposin-B; Saposin-C; Saposin-D; SAP2; Variant Gaucher Disease And Variant Metachromatic Leukodystrophy; PARK24; PSAPD |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Tris/EDTA buffer (pH 9.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity chromatography purified |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PSAP |
| Gene Full Name | Prosaposin |
| Background | This gene encodes a highly conserved preproprotein that is proteolytically processed to generate four main cleavage products including saposins A, B, C, and D. Each domain of the precursor protein is approximately 80 amino acid residues long with nearly identical placement of cysteine residues and glycosylation sites. Saposins A-D localize primarily to the lysosomal compartment where they facilitate the catabolism of glycosphingolipids with short oligosaccharide groups. The precursor protein exists both as a secretory protein and as an integral membrane protein and has neurotrophic activities. Mutations in this gene have been associated with Gaucher disease and metachromatic leukodystrophy. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. |
| Function | Saposin-A and saposin-C stimulate the hydrolysis of glucosylceramide by beta-glucosylceramidase (EC 3.2.1.45) and galactosylceramide by beta-galactosylceramidase (EC 3.2.1.46). Saposin-C apparently acts by combining with the enzyme and acidic lipid to form an activated complex, rather than by solubilizing the substrate. |
| Cellular Localization | Lysosome, Secreted |
| Calculated MW | 58 kDa |
| PTM | Disulfide bond, Glycoprotein |
