anti-PSD95 antibody [6G6]

anti-PSD95 antibody [6G6] for ICC/IF,Immunohistochemistry,Western blot and Bovine,Human,Mouse,Rat

Neuroscience antibody


Product Description

Mouse Monoclonal antibody [6G6] recognizes PSD95

Tested Reactivity Hu, Ms, Rat, Bov
Tested Application ICC/IF, IHC, WB
Specificity Detects a ~100kDa protein corresponding to the molecular mass of PSD-95 on SDS PAGE immunoblots. An additional protein of >100kDa is also detected. Additional cross-reactive bands are detected at ~75kDa and 50kDa in rat and mouse samples.
Host Mouse
Clonality Monoclonal
Clone 6G6
Isotype IgG2a
Target Name PSD95
Species Rat
Immunogen Recombinant Rat PSD-95 (NP_062567.1)
Conjugation Un-conjugated
Full Name discs, large homolog 4 (Drosophila)
Alternate Names Postsynaptic density protein 95; SAP90; PSD-95; Synapse-associated protein 90; PSD95; SAP-90; Disks large homolog 4

Application Instructions

Application Suggestion
Tested Application Dilution
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 80 kDa


Form Liquid
Purification Purification with Protein G.
Buffer PBS (pH 7.4), 50% Glycerol and 0.09% Sodium azide
Preservative 0.09% Sodium azide
Stabilizer 50% Glycerol
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database links

GeneID: 13385 Mouse DLG4

GeneID: 1742 Human DLG4

GeneID: 29495 Rat DLG4

Gene Symbol Dlg4
Gene Full Name discs, large homolog 4 (Drosophila)
Background Postsynaptic Density protein 95 (PSD95), also known as Synapse associated protein 90kDa, is a member of the membrane-associated guanylate kinase (MAGUK) family of proteins. PSD95 is a scaffolding protein and is involved in the assembly and function of the postsynaptic density complex. These family members consist of an N-terminal variable segment followed by three amino-terminal PDZ domains, an upstream SH3 domain and an inactive carboxyl-terminal guanylate kinase (GK) domain. The first and second PDZ domain localize NMDA receptors and K+ channels to synapses, and the third binds to neuroligins which are neuronal cell adhesion molecules that interact with b-neurexins and form intercellular junctions. PSD-95 also binds to neuronal nitric oxide synthase, possibly through interactions between PDZ domains present on both proteins. Thus different PDZ domains of PSD-95 might be specialized for distinct functions. PSD95 participates in synaptic targeting of AMPA receptors through an indirect manner involving Stargazin and related transmembrane AMPA receptor regulatory proteins (TARPs). The protein is implicated in experience dependent plasticity and plays an indispensable role in learning. Mutations in PSD95 are associated with autism.
Function Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B. [UniProt]
Cellular Localization Cell Membrane, Cell Junction, Synapse
Highlight Related products:
PSD95 antibodies; Anti-Mouse IgG secondary antibodies;
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Research Area Neuroscience antibody
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Images (3) Click the Picture to Zoom In

  • ARG20545 anti-PSD95 antibody [6G6] ICC/IF image

    Immunocytochemistry: Rat dissociated hippocampal neurons fixed by cold 4% paraformaldehyde/0.2% glutaraldehyde in 0.1M Sodium phosphate buffer. Sample was stained with ARG20545 anti-PSD95 antibody [6G6] at 1:1000 dilution for 12 hours at 4°C.

  • ARG20545 anti-PSD95 antibody [6G6] ICC/IF image

    Immunofluorescence: HaCaT cells. Fixation: Cold 100% methanol for 10 min at -20°C. Primary Antibody: ARG20545 anti-PSD95 antibody [6G6] at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat anti-Mouse (green) at 1:50 for 1 hour at RT.

  • ARG20545 anti-PSD95 antibody [6G6] WB image

    Western blot: Rat membrane stained with ARG20545 anti-PSD95 antibody [6G6] at 1:1000 dilution.