anti-PTPN3 / PTPH1 phospho (Ser459) antibody

anti-PTPN3 / PTPH1 phospho (Ser459) antibody for Western blot and Human,Mouse

Signaling Transduction antibody


Product Description Rabbit Polyclonal antibody recognizes PTPN3 / PTPH1 phospho (Ser459)
Tested Reactivity Hu, Ms
Predict Reactivity Bov, Dog, NHuPrm, Sheep
Tested Application WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PTPN3 / PTPH1
Antigen Species Human
Immunogen KLH-conjugated synthetic phospho-peptide around Ser459.
Conjugation Un-conjugated
Alternate Names PTPH1; EC; Protein-tyrosine phosphatase H1; Tyrosine-protein phosphatase non-receptor type 3; PTP-H1

Application Instructions

Application Suggestion
Tested Application Dilution
Application Note Specific for ~104k PTPH1 protein phosphorylated at Ser459 . Immunolabeling is blocked by preadsorption of the antibody with the phosphopeptide used as antigen but not by the corresponding dephosphopeptide. Immunolabeling is also completely eliminated by treatment with λ phosphatase.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.


Form Liquid
Purification Affinity Purified
Buffer 10 mM HEPES (pH 7.5), 150 mM NaCl, 0.1 mg/ml BSA and 50% Glycerol
Stabilizer 0.1 mg/ml BSA, 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 545622 Mouse PTPN3

GeneID: 5774 Human PTPN3

Swiss-port # A2ALK8 Mouse Tyrosine-protein phosphatase non-receptor type 3

Swiss-port # P26045 Human Tyrosine-protein phosphatase non-receptor type 3

Gene Symbol PTPH1
Gene Full Name protein tyrosine phosphatase, non-receptor type 3
Background Protein-tyrosine Phosphatase H1 (PTPH1) has recently been identified as a specific p38γ MAPK phosphatase which is mediated through PDZ interaction (Hou et al., 2010). Ras has been demonstrated to increase both p38γ and PTPH1 protein expression, and there is a coupling of increased p38γ and PTPH1 protein expression in primary colon cancer tissues (Hou et al., 2010). Phosphorylation of PTPH1 at Ser459 leads to PTPH1 stabilization, which plays an important role in Ras oncogenesis and stress response (Hou et al., 2012). Additionally, phosphorylation of PTPH1 Ser459 reveals a novel mechanism by which MAPK signals through PTPH1 to regulate cellular response (Hou et al., 2012).
Research Area Signaling Transduction antibody
Calculated MW 104 kDa

Images (1) Click the Picture to Zoom In

  • ARG52405 anti-PTPN3 / PTPH1 phospho (Ser459) antibody WB image

    Western blot: Mouse testes lysate showing specific immunolabeling of ~104 kDa PTPH1 phosphorylated at Ser459 (Control).
    Phosphospecificity is shown in the second lane (lambda-phosphatase: λ-Ptase) stained with ARG52405 anti-PTPN3 / PTPH1 phospho (Ser459) antibody.
    The blot is identical to the control except that the lysate was incubated in λ-Ptase (400 units/100 µl lysate for 30 min, RT) before being exposed to the phospho-Ser 459 PTPN3 / PTPH1 antibody.
    The immunolabeling is completely eliminated by treatment with λ-Ptase.