ARG54439

anti-PUMA antibody

anti-PUMA antibody for Western blot,ICC/IF and Human,Mouse

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes PUMA
Tested Reactivity Hu, Ms
Tested Application ICC/IF, WB
Specificity This antibody recognizes human and mouse PUMA (23kDa). A lower molecular weight band at 16kDa is detected in MOLT4 and U937 cell lysates and probably represents the PUMA- protein.
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PUMA
Antigen Species Human
Immunogen Synthetic peptide corresponding to aa 180-193 of human PUMA-alpha (accession no NP_055232). This sequence is identical in human PUMA-alpha and PUMA-beta .
Conjugation Un-conjugated
Alternate Names PUMA; p53 up-regulated modulator of apoptosis; JFY-1; JFY1; Bcl-2-binding component 3

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IFAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control K562 and NIH/3T3

Properties

Form Liquid
Purification Immunoaffinity chroma-tography
Buffer PBS (pH 7.4) and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 170770 Mouse BBC3

GeneID: 27113 Human BBC3

Swiss-port # Q96PG8 Human Bcl-2-binding component 3

Swiss-port # Q99ML1 Mouse Bcl-2-binding component 3

Gene Symbol BBC3
Gene Full Name BCL2 binding component 3
Background PUMA (p53 Upregulated Modulator of Apoptosis) is a pro-apoptotic Bcl-2 family member that encodes two BH3 domain-containing proteins, PUMA- and PUMA- . PUMA proteins bind Bcl-2, localize to mitochondria, and induce cytochrome c release and apoptosis in response to p53.
Function Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis. Isoform 3 fails to show any growth-inhibitory or apoptotic activity. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody
Calculated MW 26 kDa

Images (2) Click the Picture to Zoom In

  • ARG54439 anti-PUMA antibody WB image

    Western Blot: H:K562; M:NIH/3T3 stained with ARG54439 anti-PUMA antibody at 2 μg/ml dilution.

  • ARG54439 anti-PUMA antibody ICC/IF image

    Immunofluorescence: K562 stained with ARG54439 anti-PUMA antibody at 1 μg/ml dilution.