ARG54440

anti-PUMA antibody

anti-PUMA antibody for Western blot,Immunohistochemistry and Human

Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody

Overview

Product Description Rabbit Polyclonal antibody recognizes PUMA
Tested Reactivity Hu
Tested Application IHC, WB
Specificity This antibody recognizes human and mouse PUMA (23kDa).
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PUMA
Antigen Species Human
Immunogen Synthetic peptide corresponding to aa 2-16 of human PUMA-alpha (accession no. NP_055232). This sequence is identical in mouse PUMA.
Conjugation Un-conjugated
Alternate Names PUMA; p53 up-regulated modulator of apoptosis; JFY-1; JFY1; Bcl-2-binding component 3

Application Instructions

Application Suggestion
Tested Application Dilution
IHCAssay-dependent
WBAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control K562 and Human breast carcinoma

Properties

Form Liquid
Purification Immunoaffinity chroma-tography
Buffer PBS (pH 7.4) and 0.02% Sodium azide
Preservative 0.02% Sodium azide
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 27113 Human BBC3

Swiss-port # Q96PG8 Human Bcl-2-binding component 3

Gene Symbol BBC3
Gene Full Name BCL2 binding component 3
Background PUMA (p53 Upregulated Modulator of Apoptosis) is a pro-apoptotic Bcl-2 family member that encodes two BH3 domain-containing proteins, PUMA- and PUMA- . PUMA proteins bind Bcl-2, localize to mitochondria, and induce cytochrome c release and apoptosis in response to p53.
Function Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis. Isoform 3 fails to show any growth-inhibitory or apoptotic activity. [UniProt]
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Signaling Transduction antibody
Calculated MW 26 kDa

Images (2) Click the Picture to Zoom In

  • ARG54440 anti-PUMA antibody WB image

    Western Blot: K562 stained with ARG54440 anti-PUMA antibody at 2 μg/ml dilution.

  • ARG54440 anti-PUMA antibody IHC image

    Immunohistochemistry: human breast carcinoma stained with ARG54440 anti-PUMA antibody at 10 μg/ml dilution.