ARG10725

anti-Parvalbumin antibody [3C9]

anti-Parvalbumin antibody [3C9] for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat,Cow

Overview

Product Description Mouse Monoclonal antibody [3C9] recognizes Parvalbumin
Tested Reactivity Hu, Ms, Rat, Cow
Tested Application ICC/IF, IHC-Fr, WB
Host Mouse
Clonality Monoclonal
Clone 3C9
Isotype IgG1
Target Name Parvalbumin
Antigen Species Human
Immunogen Full-length recombinant Human Parvalbumin.
Conjugation Un-conjugated
Full Name parvalbumin
Alternate Names D22S749; Parvalbumin alpha

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:1000 - 1:5000
IHC-Fr1:1000 - 1:5000
WB1:1000 - 1:5000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 12 kDa

Properties

Form Liquid
Purification Affinity purification.
Concentration 1 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 19293 Mouse PVALB

GeneID: 25269 Rat PVALB

GeneID: 5816 Human PVALB

Gene Symbol PVALB
Gene Full Name parvalbumin
Background The protein encoded by this gene is a high affinity calcium ion-binding protein that is structurally and functionally similar to calmodulin and troponin C. The encoded protein is thought to be involved in muscle relaxation. [provided by RefSeq, Jul 2008]
Function In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions. [UniProt]

Images (4) Click the Picture to Zoom In

  • ARG10725 anti-Parvalbumin antibody [3C9] ICC/IF image

    Immunocytochemistry: Adult Rat cerebellum floating section was stained with ARG10725 anti-Parvalbumin antibody [3C9] at 1:1000 (green). Parvalbumin is prominently expressed in the dendrites and perikarya of Purkinje cells and some interneurons in the molecular layer. Blue is a DNA stain.

  • ARG10725 anti-Parvalbumin antibody [3C9] IHC-Fr image

    Immunohistochemistry: Frozen section of Rat cerebellum stained with ARG10725 anti-Parvalbumin antibody [3C9] (green) at 1:1000 dilution and costained with Chicken pAb to calbindin (red) at 1:2000 dilution. DAPI (blue) for nuclear staining. (Sample preparation: Following transcardial perfusion of Rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with above antibodies.)

    Most Purkinje cells coexpress both parvalbumin and calbindin and so appear yellow, whereas basket, stellate and Golgi cells express parvalbumin only and so appear green.

  • ARG10725 anti-Parvalbumin antibody [3C9] WB image

    Western blot: Rat skeletal muscle lysate (left) and His-tagged recombinant proteins (right): 1) parvalbumin, 2) calretinin, and 3) calbindin. Blots were stained with ARG10725 anti-Parvalbumin antibody [3C9] at 1:1000. In  skeletal muscle lysates, this antibody recognizes a band at 12 kDa which represents parvalbumin. It reacts to recombinant parvalbumin only, not the other calcium-binding proteins listed. Note, this antibody does not recognize parvabumin in Rat or mouse brain lysates on western blots. Protein from Rat skeletal muscle lysates were transferred to PVDF membrane.

  • ARG10725 anti-Parvalbumin antibody [3C9] IHC-Fr image

    Immunohistochemistry: Frozen section of Rat brain cerebellum stained with ARG10725 anti-Parvalbumin antibody [3C9] (green) at 1:1000 dilution and costained with Chicken pAb to calbindin (red) at 1:2000 dilution. (Sample preparation: Following transcardial perfusion of Rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with above antibodies.)

    Most Purkinje cells coexpress both parvalbumin and calbindin and so appear yellow, whereas basket, stellate and Golgi cells express parvalbumin only and so appear green.