anti-Parvalbumin antibody for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat
|Product Description||Chicken Polyclonal antibody recognizes Parvalbumin|
|Tested Reactivity||Hu, Ms, Rat|
|Tested Application||ICC/IF, IHC-Fr, WB|
|Immunogen||Full-length recombinant Human Parvalbumin.|
|Alternate Names||D22S749; Parvalbumin alpha|
|Application Note||* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.|
|Buffer||PBS and 0.02% Sodium azide.|
|Preservative||0.02% Sodium azide|
|Storage Instruction||For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.|
|Note||For laboratory research only, not for drug, diagnostic or other use.|
|Gene Full Name||parvalbumin|
|Background||The protein encoded by this gene is a high affinity calcium ion-binding protein that is structurally and functionally similar to calmodulin and troponin C. The encoded protein is thought to be involved in muscle relaxation. [provided by RefSeq, Jul 2008]|
|Function||In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions. [UniProt]|
|Calculated MW||12 kDa|
Images (3) Click the Picture to Zoom In
ARG10686 anti-Parvalbumin antibody ICC/IF image
Immunocytochemistry: Adult Rat cerebellum floating section was stained with ARG10686 anti-Parvalbumin antibody at 1:2500 (green). Parvalbumin is prominently expressed in the dendrites and perikarya of Purkinje cells and the molecular layer interneurons. Blue is a DNA stain.
ARG10686 anti-Parvalbumin antibody IHC-Fr image
Immunohistochemistry: Frozen section of Rat cerebellum stained with ARG10686 anti-Parvalbumin antibody (green) at 1:2000 dilution and costained with Mouse mAb to GFAP (red) at 1:500 dilution. DAPI (blue) for nuclear staining. (Sample preparation: Following transcardial perfusion of Rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45 µM, and free-floating sections were stained with the above antibodies.)
The Parvalbumin antibody labels the perikarya and dendrites of Purkinje cells and interneurons in the molecular layer of the cerebellum. The GFAP antibody stains the processes of Bergmann glia and astrocytes.
ARG10686 anti-Parvalbumin antibody WB image
Western blot: 1) Mouse skeletal muscle lysate, and His-tagged recombinant proteins: 2) parvalbumin, 3) calretinin, and 4) calbindin was stained with ARG10686 anti-Parvalbumin antibody at 1:2,500. In skeletal muscle lysates, this antibody recognizes a band at 12 kDa which represents parvalbumin and it reacts only with parvalbumin and not the other calcium-binding proteins. This antibody does not recognize parvabumin in Rat or mouse brain lysates on western blots.