anti-Perforin antibody [deltaG9] (PE)

anti-Perforin antibody [deltaG9] (PE) for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections and Human,Bovine

Immune System antibody


Product Description PE-conjugated Mouse Monoclonal antibody [deltaG9] recognizes Perforin
Tested Reactivity Hu, Bov
Tested Application FACS, ICC/IF, IHC-Fr, IHC-P
Specificity This antibody recognizes perforin, a 70 kDa protein expressed in cytoplasmic granules of cytotoxic T cells and NK cells.
Host Mouse
Clonality Monoclonal
Clone deltaG9
Isotype IgG2b
Target Name Perforin
Antigen Species Human
Immunogen Purified granules from human YT lymphoma cell line
Conjugation PE
Alternate Names FLH2; P1; PFN1; Cytolysin; HPLH2; PFP; Lymphocyte pore-forming protein; Perforin-1

Application Instructions

Application Suggestion
Tested Application Dilution
Application Note Flow Cytometry: for intracellular staining.


Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS, 15 mM Sodium azide and 0.2% (w/v) high-grade protease free BSA
Preservative 15 mM Sodium azide
Stabilizer 0.2% (w/v) high-grade protease free BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Keep the antibody in the dark and keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 5551 Human PRF1

Swiss-port # P14222 Human Perforin-1

Gene Symbol PRF1
Gene Full Name perforin 1 (pore forming protein)
Background Perforin with structural similarities to complement component C9 that is important in immunity. This protein forms membrane pores that allow the release of granzymes and subsequent cytolysis of target cells. Whether pore formation occurs in the plasma membrane of target cells or in an endosomal membrane inside target cells is subject to debate. Mutations in this gene are associated with a variety of human disease including diabetes, multiple sclerosis, lymphomas, autoimmune lymphoproliferative syndrome (ALPS), aplastic anemia, and familial hemophagocytic lymphohistiocytosis type 2 (FHL2), a rare and lethal autosomal recessive disorder of early childhood. [provided by RefSeq, Aug 2017]
Function Perforin plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes. [UniProt]
Highlight Related products:
Perforin antibodies; Perforin ELISA Kits; Anti-Mouse IgG secondary antibodies;
Related news:
Examining CTL/NK-mediated cytotoxicity by ELISA
Research Area Immune System antibody
Calculated MW 61 kDa
PTM N-glycosylated.

Images (1) Click the Picture to Zoom In

  • ARG54648 anti-Perforin antibody [deltaG9] (PE) FACS image

    Flow Cytometry: Human peripheral blood cells stained with ARG54648 anti-Perforin antibody [deltaG9] (PE).