ARG52393

anti-Periostin (pan) antibody

anti-Periostin (pan) antibody for Immunohistochemistry,Western blot and Chicken,Human,Mouse,Rat

Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Signaling Transduction antibody
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Overview

Product Description

Rabbit Polyclonal antibody recognizes Periostin (pan)

Tested Reactivity Hu, Ms, Rat, Chk
Predict Reactivity Amph, Bird, Fsh, Mamm
Tested Application IHC, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Periostin (pan)
Antigen Species Mouse
Immunogen Synthetic peptide corresponding to amino acid residues from the fasciclin domain 1 of mouse periostin
Conjugation Un-conjugated
Alternate Names OSF2; PN; Osteoblast-specific factor 2; Periostin; OSF-2; PDLPOSTN

Application Instructions

Application Suggestion
Tested Application Dilution
IHC1:100
WB1:1000
Application Note Specific for three periostin bands around ~93 kDa in mouse lung extract. The immunogenic peptide is present in all known splice variants of periostin and therefore this antibody recognizes all known molecular forms of periostin. The antibody also works well for immunohistochemistry on paraformaldehyde-fixed sections with a simple antigenretrieval protocol (incubate slides for 20 minutes at 90º C in 10 mM sodium citRate (pH 6.0)/ 0.1 % Tween-20).
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity Purified
Buffer PBS
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10631 Human POSTN

GeneID: 50706 Mouse POSTN

Swiss-port # Q15063 Human Periostin

Swiss-port # Q62009 Mouse Periostin

Gene Symbol POSTN
Gene Full Name periostin, osteoblast specific factor
Background Periostin is a matricellular protein, i.e. an extracellular matrix protein that interacts both with other ECM proteins and with cell-surface receptors. Like many other matricellular proteins, the function of periostin is important both in embryonic development and in the remodeling of adult tissues in response to pathological insults. Periostin was originally isolated as an osteoblast-specific marker that functions as a cell adhesion molecule for preosteoblasts and is thought to be involved in osteoblast recruitment, attachment and spreading (Kruzynska-Frejtag A. et al., 2004). Periostin has recently been shown to promote collagen fibrogenesis, inhibit differentiation of progenitor cells into cardiomyocytes and to be essential in maintaining the biomechanical properties of the adult myocardium (Norris et al., 2008)
Research Area Cancer antibody; Cell Biology and Cellular Response antibody; Controls and Markers antibody; Signaling Transduction antibody
Calculated MW 93 kDa
PTM Gamma-carboxylation is controversial. Gamma-carboxyglutamated; gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation; this may be required for calcium binding (PubMed:18450759). According to a more recent report, does not contain vitamin K-dependent gamma-carboxyglutamate residues (PubMed:26273833).

Images (1) Click the Picture to Zoom In

  • ARG52393 anti-Periostin (pan) antibody WB image

    Western blot: rat lung lysate stained with ARG52393 anti-Periostin (pan) antibody showing specific immunolabeling of the ~ 93 kDa periostin protein triplet.

Customer's Feedback

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Review for anti-Periostin (pan) antibody

Application:WB

Sample:Rat brain

Sample Loading Amount:30 µg

Primary Antibody Dilution Factor:1:1000

Primary Antibody Incubation Time:overnight

Primary Antibody Incubation Temperature:4 ºC