ARG10820

anti-Phospho-PDE5A antibody

anti-Phospho-PDE5A antibody for ELISA,ICC/IF,Immunohistochemistry,Immunoprecipitation,Western blot,Confocal microscopy (Confocal),Dot blot and Bovine,Human,Mouse,Pig,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes PDE5A
Tested Reactivity Hu, Ms, Rat, Bov, Pig
Tested Application Confocal, Dot, ELISA, ICC/IF, IHC, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name PDE5A
Species Human
Immunogen Phosphorylated peptide taken within aa. 120-170 from Human PDE5A.
Conjugation Un-conjugated
Full Name phosphodiesterase 5A, cGMP-specific
Alternate Names cGMP-specific 3',5'-cyclic phosphodiesterase; CN5A; CGB-PDE; cGMP-binding cGMP-specific phosphodiesterase; PDE5; EC 3.1.4.35

Application Instructions

Application Suggestion
Tested Application Dilution
Confocal1:100
Dot1:10000
ELISA1:10000
ICC/IF1:100
IHC1:100
IP1:200
WB1:500
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Calculated MW 100 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer Tris-Glycine Buffer (pH 7.4 - 7.8), Hepes, 0.02% Sodium azide, 30% Glycerol and 0.5% BSA.
Preservative 0.02% Sodium azide
Stabilizer 30% Glycerol and 0.5% BSA
Concentration 0.5 mg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 171115 Rat PDE5A

GeneID: 242202 Mouse PDE5A

GeneID: 281972 Bovine PDE5A

Gene Symbol PDE5A
Gene Full Name phosphodiesterase 5A, cGMP-specific
Background This gene encodes a cGMP-binding, cGMP-specific phosphodiesterase, a member of the cyclic nucleotide phosphodiesterase family. This phosphodiesterase specifically hydrolyzes cGMP to 5'-GMP. It is involved in the regulation of intracellular concentrations of cyclic nucleotides and is important for smooth muscle relaxation in the cardiovascular system. Alternative splicing of this gene results in three transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]
Function Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP. Specifically regulates nitric-oxide-generated cGMP. [UniProt]
PTM Phosphorylation is regulated by binding of cGMP to the two allosteric sites (By similarity). Phosphorylation by PRKG1 leads to its activation.

Images (1) Click the Picture to Zoom In

  • ARG10820 anti-Phospho-PDE5A antibody WB image

    Western blot: Human PDE5A-GFP overexpressing cells untreated or treated with ANP. The blots were stained with ARG10820 anti-Phospho-PDE5A antibody at 1:500 dilution.