ARG20543

anti-Phosphoserine antibody

anti-Phosphoserine antibody for ELISA,ICC/IF,Immunohistochemistry,Immunoprecipitation,Western blot and Other

Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody

Overview

Product Description

Rabbit Polyclonal antibody recognizes Phosphoserine

Tested Reactivity Other
Tested Application ELISA, ICC/IF, IHC, IP, WB
Specificity Recognizes proteins phosphorylated on serine residues. Does not cross-react with phosphotyrosine.
Host Rabbit
Clonality Polyclonal
Target Name Phosphoserine
Immunogen KLH-conjugated Phosphoserine, and phosvitin mixture
Conjugation Un-conjugated

Application Instructions

Application Suggestion
Tested Application Dilution
ELISA1:250
ICC/IF1:50
IHCAssay-dependent
IP1:100
WB1:500
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Rabbit immunoglobulin
Buffer PBS, 50% Glycerol and 0.09% Sodium azide
Preservative 0.09% Sodium azide
Stabilizer 50% Glycerol
Concentration 250 µg/ml
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Background Protein phosphorylation is an important posttranslational modification that serves many key functions to regulate a protein’s activity, localization, and protein-protein interactions. Phosphorylation is catalyzed by various specific protein kinases, which involves removing a phosphate group from ATP and covalently attaching it to to a recipient protein that acts as a substrate. Most kinases act on both serine and threonine; others act on tyrosine, and a number (dual specificity kinases) act on all three. Because phosphorylation can occur at multiple sites on any given protein, it can therefore change the function or localization of that protein at any time. Changing the function of these proteins has been linked to a number of diseases, including cancer, diabetes, heart disease, inflammation and neurological disorders.
Research Area Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody

Images (2) Click the Picture to Zoom In

  • ARG20543 anti-Phosphoserine antibody ICC/IF image

    Immunofluorescence: HeLa cells. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: ARG20543 anti-Phosphoserine antibody at 1:50 for 12 hours at 4°C. Secondary Antibody: R-PE Goat anti-Rabbit (yellow) at 1:200 for 2 hours at RT. Counterstain: DAPI (blue) nuclear stain at 1:40000 for 2 hours at RT. Magnification: 100x. Left: DAPI (blue) nuclear stain. Middle: ARG20543 anti-Phosphoserine antibody. Right: Composite.

  • ARG20543 anti-Phosphoserine antibody WB image

    Western blot: the phosphorylated proteins with UV-treated cell lysates (mouse spleen cell) stained with ARG20543 anti-Phosphoserine antibody.  Bands are responsive to treatment with varying long UV wavelengths: A(0), B(50), C(200), D(400), and E (treated with 0.1 μM okadaic acid).