ARG10687

anti-Pin 1 antibody

anti-Pin 1 antibody for Western blot,ICC/IF,IHC-Frozen sections and Human,Horse,Cow,Pig,Rat,Mouse

Overview

Product Description Chicken Polyclonal antibody recognizes Pin 1
Tested Reactivity Hu, Ms, Rat, Cow, Hrs, Pig
Predict Reactivity Chk
Tested Application ICC/IF, IHC-Fr, WB
Host Chicken
Clonality Polyclonal
Isotype IgY
Target Name Pin 1
Immunogen Recombinant full length Pin-1 purified from E. coli.
Conjugation Un-conjugated
Full Name peptidylprolyl cis/trans isomerase, NIMA-interacting 1
Alternate Names UBL5; PPIase Pin1; DOD; Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1; EC 5.2.1.8; Rotamase Pin1; Peptidyl-prolyl cis-trans isomerase Pin1

Application Instructions

Application Suggestion
Tested Application Dilution
ICC/IF1:500 - 1:1000
IHC-Fr1:500 - 1:1000
WB1:10000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Buffer PBS and 0.02% Sodium azide.
Preservative 0.02% Sodium azide
Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database links

GeneID: 23988 Mouse PIN1

GeneID: 5300 Human PIN1

Swiss-port # Q13526 Human Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1

Swiss-port # Q9QUR7 Mouse Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1

Gene Symbol PIN1
Gene Full Name peptidylprolyl cis/trans isomerase, NIMA-interacting 1
Background Peptidyl-prolyl cis/trans isomerases (PPIases) catalyze the cis/trans isomerization of peptidyl-prolyl peptide bonds. This gene encodes one of the PPIases, which specifically binds to phosphorylated ser/thr-pro motifs to catalytically regulate the post-phosphorylation conformation of its substrates. The conformational regulation catalyzed by this PPIase has a profound impact on key proteins involved in the regulation of cell growth, genotoxic and other stress responses, the immune response, induction and maintenance of pluripotency, germ cell development, neuronal differentiation, and survival. This enzyme also plays a key role in the pathogenesis of Alzheimer's disease and many cancers. Multiple alternatively spliced transcript variants have been found for this gene.[provided by RefSeq, Jun 2011]
Function Peptidyl-prolyl cis/trans isomerase (PPIase) that binds to and isomerizes specific phosphorylated Ser/Thr-Pro (pSer/Thr-Pro) motifs in a subset of proteins, resulting in conformational changes in the proteins. Displays a preference for an acidic residue N-terminal to the isomerized proline bond. Regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Down-regulates kinase activity of BTK. Can transactivate multiple oncogenes and induce centrosome amplification, chromosome instability and cell transformation. Required for the efficient dephosphorylation and recycling of RAF1 after mitogen activation. Binds and targets PML and BCL6 for degradation in a phosphorylation-dependent manner. Acts as a regulator of JNK cascade by binding to phosphorylated FBXW7, disrupting FBXW7 dimerization and promoting FBXW7 autoubiquitination and degradation: degradation of FBXW7 leads to subsequent stabilization of JUN. [UniProt]
PTM Phosphorylation at Ser-71 by DAPK1 results in inhibition of its catalytic activity, nuclear localization, and its ability to induce centrosome amplification, chromosome instability and cell transformation.

Images (2) Click the Picture to Zoom In

  • ARG10687 anti-Pin 1 antibody ICC/IF image

    Immunocytochemistry: HeLa cells stained with ARG10687 anti-Pin 1 antibody at 1:1000 dilution (green) and co-stained with a monoclonal 38F3 to fibrillarin (red). Pin-1 stains the nuclear matrix and, much more faintly, the cytoplasm. The fibrillarin antibody marks nucleoli.

  • ARG10687 anti-Pin 1 antibody WB image

    Western blot: Whole HeLa cell homogenate stained with ARG10687 anti-Pin 1 antibody at 1:10000 dilution. A prominent band running with an apparent SDS-PAGE molecular weight of ~21 kDa corresponds to Pin1.