ARG55644
anti-Prohibitin phospho (Thr258) antibody
anti-Prohibitin phospho (Thr258) antibody for Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Prohibitin phospho (Thr258) |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | WB |
| Specificity | The antibody detects endogenous level of Prohibitin only when phosphorylated at threonine 258. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Prohibitin |
| Antigen Species | Human |
| Immunogen | KLH-conjugated phosphospecific peptide around Thr258 (N-I-T(p)-Y-L) derived from Human Prohibitin |
| Conjugation | Un-conjugated |
| Alternate Names | HEL-215; PHB1; Prohibitin; HEL-S-54e |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with phospho-specific peptide and the non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. |
| Buffer | PBS (pH 7.4), 150mM NaCl, 0.02% Sodium azide and 50% Glycerol |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | PHB |
| Gene Full Name | prohibitin |
| Background | This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013] |
| Function | Prohibitin inhibits DNA synthesis. It has a role in regulating proliferation. As yet it is unclear if the protein or the mRNA exhibits this effect. May play a role in regulating mitochondrial respiration activity and in aging. [UniProt] |
| Calculated MW | 30 kDa |
Images (1) Click the Picture to Zoom In
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ARG55644 anti-Prohibitin phospho (Thr258) antibody WB image
Western blot: MCF, PC12, 293 and 3T3 cell lysates stained with ARG55644 anti-Prohibitin phospho (Thr258) antibody.
